Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells

Jer Chia Tsai, Mukesh Jain, Chung Ming Hsieh, Wen Sen Lee, Masao Yoshizumi, Cam Patterson, Mark A. Perrella, Carol Cooke, Hong Wang, Edgar Haber, Robert Schlegel, Mu En Lee

研究成果: 雜誌貢獻文章同行評審

212 引文 斯高帕斯(Scopus)

摘要

Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30% at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.

原文英語
頁(從 - 到)3667-3670
頁數4
期刊Journal of Biological Chemistry
271
發行號7
DOIs
出版狀態已發佈 - 2月 16 1996
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學

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