Increased serum interleukin-17 and peripheral Th17 cells in children with acute Henoch-Schönlein purpura

Hsiao-Yu Jen, Ya-Hui Chuang, Sheng-Chieh Lin, Bor-Luen Chiang, Yao-Hsu Yang

研究成果: 雜誌貢獻文章同行評審

29 引文 斯高帕斯(Scopus)

摘要

Background: Interleukin (IL)-17 and Th17 cells have been involved in many autoimmune diseases. The aim of this study is to investigate the involvement of IL-17 and Th17 cells in the pathogenesis of childhood Henoch-Schönlein purpura (HSP). Methods: Serum and supernatant levels of cytokines and chemokines were analyzed by enzyme-linked immunosorbent assay (ELISA). Using intracellular staining, the frequency of peripheral Th17 and Th1 cells was studied by flow cytometry. Results: Children with acute HSP had significantly higher serum levels of IL-17, IL-6 and transforming growth factor-β than healthy controls. The IL-17 levels in culture supernatants of peripheral blood mononuclear cells with anti-CD3 and CD28 antibody stimulation were much higher in patients with HSP (281.2±91.4 vs. 47.7±22.6pg/ml, p=0.022). The patients also had more Th17 cells (1.67±0.36% vs. 0.71±0.15%, p=0.033) but not Th1 cells in peripheral blood. Moreover, IL-17 could promote human endothelial cells to produce chemoattractants IL-8 and monocyte chemotactic protein-1. Conclusion: The increased frequency of peripheral Th17 cells and serum IL-17 levels are shown in childhood HSP that may in part contribute to vascular inflammation, suggesting cellular immunity is likely to be involved in the process of HSP. © 2011 John Wiley & Sons A/S.
原文英語
頁(從 - 到)862-868
頁數7
期刊Pediatric Allergy and Immunology
22
發行號8
DOIs
出版狀態已發佈 - 2011

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