Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS

Chu Chun Huang, Chia Hung Chou, Shee Uan Chen, Hong Nerng Ho, Yu Shih Yang, Mei Jou Chen

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Background/Purpose: Abnormal folliculogenesis is one of the cardinal presentations of polycystic ovarian syndrome (PCOS) and permeability of follicular wall has been proposed to be involved in the normal follicular growth. However, whether or not there is a change in intrafollicular permeability underlies PCOS is unknown. Methods: This was a tertiary center-based case–control study. From 2014 to 2015, thirteen patients with PCOS who underwent in vitro fertilization-embryo transfer (IVF-ET) were enrolled. Eleven normo-ovulatory patients who underwent IVF-ET due to male factor and/or tubal factor infertility were enrolled as the control group. The influence of ovarian follicular fluid (FF) on endothelial cell permeability was evaluated using a human umbilical vein endothelial cell monolayer permeability assay. The intrafollicular expression profiles of angiogenesis-related proteins were analyzed using a Human Angiogenesis Protein Array Kit. Results: The FF from PCOS patients caused significantly poorer endothelial cell permeability comparing with the effect of FF from the control group (46% ± 12% vs. 58% ± 9%, P = 0.023). Among the 55 angiogenesis-related proteins tested, there was a significantly higher level of intrafollicular platelet factor 4 (PF4) and PF4/IL-8 complex in the PCOS group (p = 0.004). The anti-permeability effect of PF4 was related to the decrease in the intercellular gaps and antagonistic binding with IL-8. Conclusion: Our study provides the first evidence of the pathophysiologic contribution of the well-known angiostatic protein, PF4, on human reproductive biology. The increase of the intrafollicular PF4 and its anti-permeability effect might affect the formation of FF and folliculogenesis in PCOS.
原文英語
頁(從 - 到)249-259
頁數11
期刊Journal of the Formosan Medical Association
118
發行號1P2
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Platelet Factor 4
Follicular Fluid
Polycystic Ovary Syndrome
Permeability
Embryo Transfer
Fertilization in Vitro
Interleukin-8
Angiostatic Proteins
Endothelial Cells
Protein Array Analysis
Control Groups
Human Umbilical Vein Endothelial Cells
Infertility
Proteins
Growth

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS. / Huang, Chu Chun; Chou, Chia Hung; Chen, Shee Uan; Ho, Hong Nerng; Yang, Yu Shih; Chen, Mei Jou.

於: Journal of the Formosan Medical Association, 卷 118, 編號 1P2, 01.01.2019, p. 249-259.

研究成果: 雜誌貢獻文章

Huang, Chu Chun ; Chou, Chia Hung ; Chen, Shee Uan ; Ho, Hong Nerng ; Yang, Yu Shih ; Chen, Mei Jou. / Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS. 於: Journal of the Formosan Medical Association. 2019 ; 卷 118, 編號 1P2. 頁 249-259.
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abstract = "Background/Purpose: Abnormal folliculogenesis is one of the cardinal presentations of polycystic ovarian syndrome (PCOS) and permeability of follicular wall has been proposed to be involved in the normal follicular growth. However, whether or not there is a change in intrafollicular permeability underlies PCOS is unknown. Methods: This was a tertiary center-based case–control study. From 2014 to 2015, thirteen patients with PCOS who underwent in vitro fertilization-embryo transfer (IVF-ET) were enrolled. Eleven normo-ovulatory patients who underwent IVF-ET due to male factor and/or tubal factor infertility were enrolled as the control group. The influence of ovarian follicular fluid (FF) on endothelial cell permeability was evaluated using a human umbilical vein endothelial cell monolayer permeability assay. The intrafollicular expression profiles of angiogenesis-related proteins were analyzed using a Human Angiogenesis Protein Array Kit. Results: The FF from PCOS patients caused significantly poorer endothelial cell permeability comparing with the effect of FF from the control group (46{\%} ± 12{\%} vs. 58{\%} ± 9{\%}, P = 0.023). Among the 55 angiogenesis-related proteins tested, there was a significantly higher level of intrafollicular platelet factor 4 (PF4) and PF4/IL-8 complex in the PCOS group (p = 0.004). The anti-permeability effect of PF4 was related to the decrease in the intercellular gaps and antagonistic binding with IL-8. Conclusion: Our study provides the first evidence of the pathophysiologic contribution of the well-known angiostatic protein, PF4, on human reproductive biology. The increase of the intrafollicular PF4 and its anti-permeability effect might affect the formation of FF and folliculogenesis in PCOS.",
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AU - Huang, Chu Chun

AU - Chou, Chia Hung

AU - Chen, Shee Uan

AU - Ho, Hong Nerng

AU - Yang, Yu Shih

AU - Chen, Mei Jou

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AB - Background/Purpose: Abnormal folliculogenesis is one of the cardinal presentations of polycystic ovarian syndrome (PCOS) and permeability of follicular wall has been proposed to be involved in the normal follicular growth. However, whether or not there is a change in intrafollicular permeability underlies PCOS is unknown. Methods: This was a tertiary center-based case–control study. From 2014 to 2015, thirteen patients with PCOS who underwent in vitro fertilization-embryo transfer (IVF-ET) were enrolled. Eleven normo-ovulatory patients who underwent IVF-ET due to male factor and/or tubal factor infertility were enrolled as the control group. The influence of ovarian follicular fluid (FF) on endothelial cell permeability was evaluated using a human umbilical vein endothelial cell monolayer permeability assay. The intrafollicular expression profiles of angiogenesis-related proteins were analyzed using a Human Angiogenesis Protein Array Kit. Results: The FF from PCOS patients caused significantly poorer endothelial cell permeability comparing with the effect of FF from the control group (46% ± 12% vs. 58% ± 9%, P = 0.023). Among the 55 angiogenesis-related proteins tested, there was a significantly higher level of intrafollicular platelet factor 4 (PF4) and PF4/IL-8 complex in the PCOS group (p = 0.004). The anti-permeability effect of PF4 was related to the decrease in the intercellular gaps and antagonistic binding with IL-8. Conclusion: Our study provides the first evidence of the pathophysiologic contribution of the well-known angiostatic protein, PF4, on human reproductive biology. The increase of the intrafollicular PF4 and its anti-permeability effect might affect the formation of FF and folliculogenesis in PCOS.

KW - Angiogenesis

KW - Folliculogenesis

KW - Permeability

KW - Platelet factor 4

KW - Polycystic ovarian syndrome

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