Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid

Shyun Y. Liu, Mei Huei Lin, Shun Chun Yang, Guan Cheng Huang, Lisa Chang, Sam Chang, Ching Y. Yen, Wei F. Chiang, Yung Y. Kuo, Li L. Chen, Chin H. Lee, Young Chau Liu

研究成果: 雜誌貢獻文章同行評審

18 引文 斯高帕斯(Scopus)

摘要

Background and Purpose: Arecoline, an areca quid (AQ) component, has been shown to inhibit the secretion and activity of matrix metalloproteinase-2 (MMP-2) in fibroblast cultures. This study assessed whether MMP-2 expression was inhibited in the saliva samples and tumor specimens of oral tumor patients with a long-term history of AQ consumption. The net effect of crude AQ extract (AQE) on MMP-2 expression by oral cells was also investigated. Methods: Western blot analysis, zymography, and reverse transcriptase-polymerase chain reaction were used to detect MMP-2 protein and mRNA in saliva and tumor samples, as well as in the conditioned media (CM) of oral cell cultures. Results: The level of MMP-2 protein was significantly higher in the saliva samples of 12 oral tumor patients who had a minimum 10-year AQ-consuming history than in those of 12 non-AQ-using healthy controls (p <0.05). MMP-2 mRNA was expressed in 26 of 28 oral squamous cell carcinoma (OSCC) specimens. MMP-2 protein was also detectable in the tested OSC-C homogenates. Short-term stimulation with 10% AQE increased the secretion of MMP-2 protein in the CM of oral epidermoid carcinoma cell Meng-1 (an OSCC cell line) and oral fibroblasts. Conclusions: MMP-2 expression is elevated rather than inhibited in most oral tumor patients with long-term AQ usage. Short-term AQE stimulation also increases the secretion of MMP-2 by oral epithelial cells and fibroblasts. Our results suggest that AQ consumption may promote oral tumor progression through the induction of MMP-2 secretion.
原文英語
頁(從 - 到)390-397
頁數8
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
104
發行號6
出版狀態已發佈 - 2005
對外發佈

ASJC Scopus subject areas

  • 醫藥 (全部)

指紋

深入研究「Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid」主題。共同形成了獨特的指紋。

引用此