Increased dengue virus-infected endothelial cell apoptosis caused by antibodies against nonstructural protein 1

Chiou Feng Lin, Shu Wen Wan, Mei Chun Chen, Huan Yao Lei, Yee Shin Lin

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Vascular dysfunction is a hallmark of severe dengue haemorrhagic fever (DHF) in dengue virus (DENV) infection. Both viral infection and immunopathogenesis are involved in the vasculopathy of DHF. We previously showed that antibodies against DENV nonstructural protein 1 (NS1) may cross-react with endothelial cells. A further study demonstrated the pathogenic role of anti-NS1 antibodies on endothelial dysfunction by inducing cell death and inflammation. In this study, we investigated the effect of anti-NS1 antibodies on DENV-infected human endothelial cells. We found increased binding activity of anti-NS1 antibodies to endothelial cells after DENV infection. Either DENV infection or anti-NS1 treatment caused endothelial cell apoptosis. Importantly, co-treatment with anti-NS1 antibodies increased DENV-induced endothelial cell apoptosis. The generation of nitric oxide (NO) could be detected in anti-NS1-stimulated, but not DENV-infected, endothelial cells. Furthermore, anti-NS1-induced endothelial cell apoptosis was NO-dependent, whereas DENV infection-induced apoptosis was NO-independent. These results suggest an additive effect but distinct mechanisms between anti-NS1 antibodies and DENV in endothelial cell damage. These findings indicate that both viral infection and cross-reactive antibodies may be involved in dengue pathogenesis by causing endothelial dysfunction.

原文英語
頁(從 - 到)111-117
頁數7
期刊Dengue Bulletin
31
出版狀態已發佈 - 12月 2007
對外發佈

ASJC Scopus subject areas

  • 傳染性疾病
  • 病毒學

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