Increased circulating fibrocytes in asthma with chronic airflow obstruction

Chun H. Wang, Chien D. Huang, Horng Chyuan Lin, Kang Y. Lee, Shu M. Lin, Chien Ying Liu, Kuo Hsiung Huang, Yu Shien Ko, Fan Chung Kian, Han P. Kuo

研究成果: 雜誌貢獻文章

122 引文 (Scopus)

摘要

Rationale: A proportion of patients with asthma present with chronic airflow obstruction (CAO). We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall. Objectives: To compare the proportion, proliferation, and differentiation of circulating fibrocytes from patients with asthma with CAO or no airflow obstruction (NOA) and control subjects. Methods: We investigated circulating fibrocytes in 11 patients with asthma with CAO and a rapid decline in FEV1, 9 patients with asthma with NOA, and 10 nonasthmatic control subjects. Blood nonadherent non-T (NANT) cells were incubated with fetal calf serum or each patient's own serum and fibrocytes expressing CD34, CD45, and collagen I with α-smooth muscle actin were identified by flow cytometry. Measurements and Main Results: A higher percentage of circulating fibrocytes in NANT cells was found in patients with CAO when compared with patients with NOA and control subjects. In CAO, the slope of the yearly decline in FEV1 correlated with circulating fibrocytes (r = -0.756, n = 11, P <0.01). When NANT cells from patients with CAO were cultured in the patients' own sera, more fibrocytes were detected than when cultured in sera from patients with NOA or from normal subjects. An anti-transforming growth factor (TGF)-β1-neutralizing antibody inhibited α-smooth muscle actin-positive fibrocyte transformation from NANT cells of patients with CAO. Serum TGF-β1 levels were higher in patients with CAO than in patients with NOA or in normal subjects. Conclusions: Circulating fibrocytes are increased in patients with asthma with CAO and can be transformed by TGF-β1 to myofibroblasts. Fibrocytes may contribute to airway obstruction in asthma.
原文英語
頁(從 - 到)583-591
頁數9
期刊American Journal of Respiratory and Critical Care Medicine
178
發行號6
DOIs
出版狀態已發佈 - 九月 15 2008
對外發佈Yes

指紋

Chronic Obstructive Pulmonary Disease
Asthma
Transforming Growth Factors
Serum
Smooth Muscle
Actins
Myofibroblasts
Airway Obstruction
Neutralizing Antibodies
Flow Cytometry
Collagen
Stem Cells
Fibroblasts

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

引用此文

Increased circulating fibrocytes in asthma with chronic airflow obstruction. / Wang, Chun H.; Huang, Chien D.; Lin, Horng Chyuan; Lee, Kang Y.; Lin, Shu M.; Liu, Chien Ying; Huang, Kuo Hsiung; Ko, Yu Shien; Kian, Fan Chung; Kuo, Han P.

於: American Journal of Respiratory and Critical Care Medicine, 卷 178, 編號 6, 15.09.2008, p. 583-591.

研究成果: 雜誌貢獻文章

Wang, Chun H. ; Huang, Chien D. ; Lin, Horng Chyuan ; Lee, Kang Y. ; Lin, Shu M. ; Liu, Chien Ying ; Huang, Kuo Hsiung ; Ko, Yu Shien ; Kian, Fan Chung ; Kuo, Han P. / Increased circulating fibrocytes in asthma with chronic airflow obstruction. 於: American Journal of Respiratory and Critical Care Medicine. 2008 ; 卷 178, 編號 6. 頁 583-591.
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abstract = "Rationale: A proportion of patients with asthma present with chronic airflow obstruction (CAO). We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall. Objectives: To compare the proportion, proliferation, and differentiation of circulating fibrocytes from patients with asthma with CAO or no airflow obstruction (NOA) and control subjects. Methods: We investigated circulating fibrocytes in 11 patients with asthma with CAO and a rapid decline in FEV1, 9 patients with asthma with NOA, and 10 nonasthmatic control subjects. Blood nonadherent non-T (NANT) cells were incubated with fetal calf serum or each patient's own serum and fibrocytes expressing CD34, CD45, and collagen I with α-smooth muscle actin were identified by flow cytometry. Measurements and Main Results: A higher percentage of circulating fibrocytes in NANT cells was found in patients with CAO when compared with patients with NOA and control subjects. In CAO, the slope of the yearly decline in FEV1 correlated with circulating fibrocytes (r = -0.756, n = 11, P <0.01). When NANT cells from patients with CAO were cultured in the patients' own sera, more fibrocytes were detected than when cultured in sera from patients with NOA or from normal subjects. An anti-transforming growth factor (TGF)-β1-neutralizing antibody inhibited α-smooth muscle actin-positive fibrocyte transformation from NANT cells of patients with CAO. Serum TGF-β1 levels were higher in patients with CAO than in patients with NOA or in normal subjects. Conclusions: Circulating fibrocytes are increased in patients with asthma with CAO and can be transformed by TGF-β1 to myofibroblasts. Fibrocytes may contribute to airway obstruction in asthma.",
keywords = "Airway remodeling, Asthma, Fibrocytes, Myofibroblasts, Transforming growth factor-β",
author = "Wang, {Chun H.} and Huang, {Chien D.} and Lin, {Horng Chyuan} and Lee, {Kang Y.} and Lin, {Shu M.} and Liu, {Chien Ying} and Huang, {Kuo Hsiung} and Ko, {Yu Shien} and Kian, {Fan Chung} and Kuo, {Han P.}",
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T1 - Increased circulating fibrocytes in asthma with chronic airflow obstruction

AU - Wang, Chun H.

AU - Huang, Chien D.

AU - Lin, Horng Chyuan

AU - Lee, Kang Y.

AU - Lin, Shu M.

AU - Liu, Chien Ying

AU - Huang, Kuo Hsiung

AU - Ko, Yu Shien

AU - Kian, Fan Chung

AU - Kuo, Han P.

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Y1 - 2008/9/15

N2 - Rationale: A proportion of patients with asthma present with chronic airflow obstruction (CAO). We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall. Objectives: To compare the proportion, proliferation, and differentiation of circulating fibrocytes from patients with asthma with CAO or no airflow obstruction (NOA) and control subjects. Methods: We investigated circulating fibrocytes in 11 patients with asthma with CAO and a rapid decline in FEV1, 9 patients with asthma with NOA, and 10 nonasthmatic control subjects. Blood nonadherent non-T (NANT) cells were incubated with fetal calf serum or each patient's own serum and fibrocytes expressing CD34, CD45, and collagen I with α-smooth muscle actin were identified by flow cytometry. Measurements and Main Results: A higher percentage of circulating fibrocytes in NANT cells was found in patients with CAO when compared with patients with NOA and control subjects. In CAO, the slope of the yearly decline in FEV1 correlated with circulating fibrocytes (r = -0.756, n = 11, P <0.01). When NANT cells from patients with CAO were cultured in the patients' own sera, more fibrocytes were detected than when cultured in sera from patients with NOA or from normal subjects. An anti-transforming growth factor (TGF)-β1-neutralizing antibody inhibited α-smooth muscle actin-positive fibrocyte transformation from NANT cells of patients with CAO. Serum TGF-β1 levels were higher in patients with CAO than in patients with NOA or in normal subjects. Conclusions: Circulating fibrocytes are increased in patients with asthma with CAO and can be transformed by TGF-β1 to myofibroblasts. Fibrocytes may contribute to airway obstruction in asthma.

AB - Rationale: A proportion of patients with asthma present with chronic airflow obstruction (CAO). We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall. Objectives: To compare the proportion, proliferation, and differentiation of circulating fibrocytes from patients with asthma with CAO or no airflow obstruction (NOA) and control subjects. Methods: We investigated circulating fibrocytes in 11 patients with asthma with CAO and a rapid decline in FEV1, 9 patients with asthma with NOA, and 10 nonasthmatic control subjects. Blood nonadherent non-T (NANT) cells were incubated with fetal calf serum or each patient's own serum and fibrocytes expressing CD34, CD45, and collagen I with α-smooth muscle actin were identified by flow cytometry. Measurements and Main Results: A higher percentage of circulating fibrocytes in NANT cells was found in patients with CAO when compared with patients with NOA and control subjects. In CAO, the slope of the yearly decline in FEV1 correlated with circulating fibrocytes (r = -0.756, n = 11, P <0.01). When NANT cells from patients with CAO were cultured in the patients' own sera, more fibrocytes were detected than when cultured in sera from patients with NOA or from normal subjects. An anti-transforming growth factor (TGF)-β1-neutralizing antibody inhibited α-smooth muscle actin-positive fibrocyte transformation from NANT cells of patients with CAO. Serum TGF-β1 levels were higher in patients with CAO than in patients with NOA or in normal subjects. Conclusions: Circulating fibrocytes are increased in patients with asthma with CAO and can be transformed by TGF-β1 to myofibroblasts. Fibrocytes may contribute to airway obstruction in asthma.

KW - Airway remodeling

KW - Asthma

KW - Fibrocytes

KW - Myofibroblasts

KW - Transforming growth factor-β

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