Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells

Cheng Jeng Tai, Shing Chuan Shen, Woan Ruoh Lee, Ching Fong Liao, Win Ping Deng, Hung Yi Chiou, Cheng I. Hsieh, Jai Nien Tung, Ching Shyang Chen, Jeng Fong Chiou, Li Tzu Li, Chuang Yu Lin, Chung Huei Hsu, Ming Chung Jiang

研究成果: 雜誌貢獻文章

34 引文 斯高帕斯(Scopus)

摘要

Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.
原文英語
頁(從 - 到)2969-2981
頁數13
期刊Experimental Cell Research
316
發行號17
DOIs
出版狀態已發佈 - 十月 2010

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

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