TY - JOUR
T1 - Increase of oxidative stress in human sperm with lower motility
AU - Kao, Shu Huei
AU - Chao, Hsiang Tai
AU - Chen, Haw Wen
AU - Huang, Yi-Sheng
AU - Liao, Tien Ling
AU - Wei, Yau Huei
N1 - Funding Information:
Supported by research grants (NSC89-2320-B-038-082 and NSC94-2321-B-010-004-YC) from the National Science Council and the “Aim for the Top University plan” sponsored by the Ministry of Education, Taiwan, Republic of China.
PY - 2008/5
Y1 - 2008/5
N2 - Objective: To investigate the causal role of oxidative-stress status on human sperm motility. Design: To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity. Setting: University hospital infertility center. Patient(s): Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile males. Intervention(s): The levels of oxidative damage (8-hydroxy-2′-deoxyguanosine [8-OHdG] and lipid peroxides) and antioxidants (retinol, α-tocopherol, ascorbate, and protein thiols) in the spermatozoa and/or seminal plasma were measured. Main Outcome Measure(s): We analyzed the specific content of 8-OHdG and lipid peroxides by using high-performance liquid chromatography (HPLC)-electrochemical detection and HPLC-fluorescence analysis, respectively. Retinol and α-tocopherol were analyzed by using an HPLC system, whereas ascorbate and protein thiols were determined by using spectrophotometry. 8-Hydroxy-2′-deoxyguanosine was visualized by immunofluorescent staining with an anti-8-OHdG antibody that was conjugated with fluorescein isothiocyanate conjugate. Lipid peroxides in spermatozoa were stained with a fluorescent dye, C11-BODIPY581/591. Result(s): Statistically significant negative correlations were revealed between sperm motility and 8-OHdG and between motility and lipid peroxides. Statistically significant positive correlations were found between sperm motility and the levels of retinol, α-tocopherol, ascorbate, and protein thiols of seminal plasma. 8-Hydroxy-2′-deoxyguanosine and lipid peroxides in spermatozoa were found to be present mostly in mitochondria. Conclusion(s): Oxidative stress and oxidative damage were increased significantly in spermatozoa with declined motility, and the antioxidant capacities in the spermatozoa and seminal plasma were lower in males who had infertility or subfertility.
AB - Objective: To investigate the causal role of oxidative-stress status on human sperm motility. Design: To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity. Setting: University hospital infertility center. Patient(s): Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile males. Intervention(s): The levels of oxidative damage (8-hydroxy-2′-deoxyguanosine [8-OHdG] and lipid peroxides) and antioxidants (retinol, α-tocopherol, ascorbate, and protein thiols) in the spermatozoa and/or seminal plasma were measured. Main Outcome Measure(s): We analyzed the specific content of 8-OHdG and lipid peroxides by using high-performance liquid chromatography (HPLC)-electrochemical detection and HPLC-fluorescence analysis, respectively. Retinol and α-tocopherol were analyzed by using an HPLC system, whereas ascorbate and protein thiols were determined by using spectrophotometry. 8-Hydroxy-2′-deoxyguanosine was visualized by immunofluorescent staining with an anti-8-OHdG antibody that was conjugated with fluorescein isothiocyanate conjugate. Lipid peroxides in spermatozoa were stained with a fluorescent dye, C11-BODIPY581/591. Result(s): Statistically significant negative correlations were revealed between sperm motility and 8-OHdG and between motility and lipid peroxides. Statistically significant positive correlations were found between sperm motility and the levels of retinol, α-tocopherol, ascorbate, and protein thiols of seminal plasma. 8-Hydroxy-2′-deoxyguanosine and lipid peroxides in spermatozoa were found to be present mostly in mitochondria. Conclusion(s): Oxidative stress and oxidative damage were increased significantly in spermatozoa with declined motility, and the antioxidant capacities in the spermatozoa and seminal plasma were lower in males who had infertility or subfertility.
KW - Spermatozoa
KW - antioxidant capacity
KW - motility
KW - oxidative stress
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U2 - 10.1016/j.fertnstert.2007.05.029
DO - 10.1016/j.fertnstert.2007.05.029
M3 - Article
C2 - 17669405
AN - SCOPUS:43449117768
VL - 89
SP - 1183
EP - 1190
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5
ER -