Increase diagnostic efficacy by combined use of fingerprint markers in mass spectrometry-Plasma peptidomes from nasopharyngeal cancer patients for example

Joseph Tung Chieh Chang, Li Chiu Chen, Shang Yi Wei, Yin Ju Chen, Hung Ming Wang, Chun Ta Liao, I. How Chen, Ann Joy Cheng

研究成果: 雜誌貢獻文章同行評審

28 引文 斯高帕斯(Scopus)

摘要

Objectives: There is no plasma marker for detecting nasopharyngeal cancer (NPC). We developed a bead-based affinity fractionated proteomic method to search potential plasma markers for NPC. Design and methods: Affinity purification of heparinized plasma with Cu-chelated beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential NPC markers. We compiled MS protein profiles for 47 patients with NPC and compared them to profiles from 28 healthy controls. The spectra were statistically analyzed using flexAnalysis™ and ClinProt™ bioinformatics software. Diagnostic efficacy was evaluated by determination of the assay sensitivity and specificity of each marker. Results: Twelve mass fingerprint markers differing between cancer and control spectra were found. The sensitivities of these NPC markers are various ranging from 36% to 83%, and the specificities were all over 90%. Combine use of these markers significantly increases diagnostic efficacy. In which, the combined markers (2020 Da and 4635 Da) possess best discriminator with high sensitivity (94%) and specificity (93%). We further identify a C3 fragment, C3f, that may serve as a biomarker for NPC. Conclusion: The combined use of mass fingerprint markers in plasma proteome will enhance diagnostic efficacy for NPC. This method can be applied to search for novel plasma markers for cancers.

原文英語
頁(從 - 到)1144-1151
頁數8
期刊Clinical Biochemistry
39
發行號12
DOIs
出版狀態已發佈 - 十二月 2006
對外發佈Yes

ASJC Scopus subject areas

  • Clinical Biochemistry

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