Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast

Haiyan Qi, Tsai Kun Li, Debbie Kuo, Alam Nur-E-Kamal, Leroy-Fong Liu

研究成果: 雜誌貢獻文章

55 引文 斯高帕斯(Scopus)

摘要

Inactivation of the budding yeast telomere binding protein Cdc13 results in abnormal telomeres (exposed long G-strands) and activation of the DNA damage checkpoint. In the current study, we show that inactivation of Cdc13p induces apoptotic signals in yeast, as evidenced by caspase activation, increased reactive oxygen species production, and flipping of phosphatidylserine in the cytoplasmic membrane. These apoptotic signals were suppressed in a mitochondrial (ρ°) mutant. Moreover, mitochondrial proteins (e.g. MTCO3) were identified as multicopy suppressors of cdc13-1, suggesting the involvement of mitochondrial functions in telomere-initiated apoptotic signaling. These telomere-initiated apoptotic signals were also shown to depend on MEC1, but not TEL1, and were antagonized by MRE11. Our results are consistent with a model in which single-stranded G-tails in the cdc13-1 mutant trigger MEC1-dependent apoptotic signaling in yeast.

原文英語
頁(從 - 到)15136-15141
頁數6
期刊Journal of Biological Chemistry
278
發行號17
DOIs
出版狀態已發佈 - 四月 25 2003
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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