In vivo phosphorylation of titin and nebulin in frog skeletal muscle

Laura L. Somerville, Kuan Wang

研究成果: 雜誌貢獻文章

24 引文 斯高帕斯(Scopus)

摘要

Titin and nebulin are two major protein components of the sarcomere matrix in striated muscles. Purified titin and nebulin from frog (Xenopus laevis) skeletal muscle are similar in size and in amino acid composition to their mammalian or avian counterparts. Both proteins contain substantial amounts of protein-bound phosphate: about 5 to 6 per titin subunit and 8 to 9 per nebulin subunit. Injection of radioactive inorganic phosphate into the dorsal lymph sacs of Xenopus laevis resulted in the significant incorporation of radioactivity into titin and nebulin within three days of incubation. Purified titin from in vivo labeled frog gastrocnemius muscle contains one mole of radioactive phosphoserine per mole of titin subunit. These data indicate that phosphorylation of frog titin and nebulin occurs in vivo.

原文英語
頁(從 - 到)986-992
頁數7
期刊Biochemical and Biophysical Research Communications
147
發行號3
DOIs
出版狀態已發佈 - 九月 30 1987
對外發佈Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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