In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium

Yao Yuan Hsieh, Chich Sheng Lin, Yu Ling Sun, Chi Chen Chang, Horng Der Tsai, Jackson Chieh Hsi Wu

研究成果: 雜誌貢獻文章同行評審

14 引文 斯高帕斯(Scopus)

摘要

Purpose: Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium. Materials and Methods: Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection. Results: LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2, LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32. Conclusions: Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.

原文英語
頁(從 - 到)79-83
頁數5
期刊Journal of Assisted Reproduction and Genetics
19
發行號2
DOIs
出版狀態已發佈 - 2002

ASJC Scopus subject areas

  • 生殖醫學
  • 遺傳學
  • 婦產科
  • 發展生物學
  • 遺傳學(臨床)

指紋

深入研究「In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium」主題。共同形成了獨特的指紋。

引用此