In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy

Hsiung Lin Tu, Yu Shen Lin, Hsia Yu Lin, Yann Hung, Leu Wei Lo, Yang Fang Chen, Chung Yuan Mou

研究成果: 雜誌貢獻文章同行評審

188 引文 斯高帕斯(Scopus)

摘要

A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.

原文英語
頁(從 - 到)172-177
頁數6
期刊Advanced Materials
21
發行號2
DOIs
出版狀態已發佈 - 1月 12 2009
對外發佈

ASJC Scopus subject areas

  • 材料科學(全部)
  • 材料力學
  • 機械工業

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