In vitro Penetration and in vivo distribution of honokiol into the intervertebral disc in rat

Chia Hsien Chen, Chang-Jung Chiang, Lien Chen Wu, Chih Hong Yang, Yi-Jie Kuo, Tung Hu Tsai

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Honokiol is a potential candidate for the treatment of intervertebral disc (IVD) degeneration. In this study, we develop in vitro and in vivo methods to detect the distribution of honokiol in intervertebral discs using high-performance liquid chromatography. A rat tail disc was used for both experimental models. For the in vivo animal experiment, blood samples and tail discs were collected at 15, 30, 60, 120 and 240 min after honokiol administration (30 mg/kg, i.v.). The analyte was separated by a mobile phase of methanol and 10 mM NaH2PO4 buffer at pH 2.8 (78:22, v/v) and pumped through a reversed-phase analytical column (250 × 4.6 mm, particle size 5 μm) at room temperature. The in vitro experimental results demonstrated that honokiol diffused into the intervertebral disc and was concentration-dependent. The active concentration is obtained for the therapeutic level at 15 and 30 min after honokiol administration in the in vivo model.
原文英語
頁(從 - 到)1297-1302
頁數6
期刊Analytical Sciences
31
發行號12
DOIs
出版狀態已發佈 - 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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