摘要
Cuphiin D1 (CD1), a macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. Moreover, the antitumor effects of CD1 are not only related to its cytotoxicity to carcinoma cell lines, but also depend on host-mediated mechanisms. In the present study, CD1 was investigated for its effects on the proliferation and cytokine secretion of human peripheral blood mononuclear cells (PBMCs). At concentrations of from 6.25 to 50 μg/ml, it enhanced the 3H-thymidine incorporation of concanavalin A (Con A)-stimulated PBMCs in a dose-dependent manner. Excretion of IL-1β, IL-2 and TNF-α by CD1-stimulated PBMCs was markedly increased in a dose-dependent manner. The results show that CD1 could stimulate PBMCs release of IL-1β, IL-2 and TNF-α and then activate T cells. Therefore, CD1-activated T cells via IL-1β in vitro might account for the host-mediated CD1 mechanism of action.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 4233-4236 |
| 頁數 | 4 |
| 期刊 | Anticancer Research |
| 卷 | 22 |
| 發行號 | 6 C |
| 出版狀態 | 已發佈 - 十一月 2002 |
指紋
ASJC Scopus subject areas
- Cancer Research
- Oncology
引用此文
In vitro immunomodulatory effects of cuphiin D1 on human mononuclear cells. / Wang, Ching Chiung; Chen, Lih Geeng; Yang, Ling-Ling.
於: Anticancer Research, 卷 22, 編號 6 C, 11.2002, p. 4233-4236.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - In vitro immunomodulatory effects of cuphiin D1 on human mononuclear cells
AU - Wang, Ching Chiung
AU - Chen, Lih Geeng
AU - Yang, Ling-Ling
PY - 2002/11
Y1 - 2002/11
N2 - Cuphiin D1 (CD1), a macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. Moreover, the antitumor effects of CD1 are not only related to its cytotoxicity to carcinoma cell lines, but also depend on host-mediated mechanisms. In the present study, CD1 was investigated for its effects on the proliferation and cytokine secretion of human peripheral blood mononuclear cells (PBMCs). At concentrations of from 6.25 to 50 μg/ml, it enhanced the 3H-thymidine incorporation of concanavalin A (Con A)-stimulated PBMCs in a dose-dependent manner. Excretion of IL-1β, IL-2 and TNF-α by CD1-stimulated PBMCs was markedly increased in a dose-dependent manner. The results show that CD1 could stimulate PBMCs release of IL-1β, IL-2 and TNF-α and then activate T cells. Therefore, CD1-activated T cells via IL-1β in vitro might account for the host-mediated CD1 mechanism of action.
AB - Cuphiin D1 (CD1), a macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. Moreover, the antitumor effects of CD1 are not only related to its cytotoxicity to carcinoma cell lines, but also depend on host-mediated mechanisms. In the present study, CD1 was investigated for its effects on the proliferation and cytokine secretion of human peripheral blood mononuclear cells (PBMCs). At concentrations of from 6.25 to 50 μg/ml, it enhanced the 3H-thymidine incorporation of concanavalin A (Con A)-stimulated PBMCs in a dose-dependent manner. Excretion of IL-1β, IL-2 and TNF-α by CD1-stimulated PBMCs was markedly increased in a dose-dependent manner. The results show that CD1 could stimulate PBMCs release of IL-1β, IL-2 and TNF-α and then activate T cells. Therefore, CD1-activated T cells via IL-1β in vitro might account for the host-mediated CD1 mechanism of action.
KW - Cuphea hyssopifolia
KW - Cuphiin D1
KW - Human peripheral blood mononuclear cells
KW - IL-1β
KW - Macrocyclic hydrolyzable tannin
UR - http://www.scopus.com/inward/record.url?scp=0036876006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036876006&partnerID=8YFLogxK
M3 - Article
C2 - 12553063
AN - SCOPUS:0036876006
VL - 22
SP - 4233
EP - 4236
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 6 C
ER -