In vitro cell growth stimulated by recombinant human cytokines can help to diagnose transient leukemia in neonates

Hsi C. Liu, Shu Huey Chen, Lin Y. Wang, Ting C. Yeh, I. J. Chai, Der Cherng Liang

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

Background/Purpose: In a previous study, we demonstrated that in vitro cell growth stimulated by human placental conditioned medium distinguished between transient leukemia (TL) and congenital acute myeloid leukemia (AML) in neonates. We then sought to determine whether the application can be expanded if in vitro cell growths are stimulated by recombinant human cytokines including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3), stem cell factor (rhSCF) anal thrombopoietin (rhTPO). Methods: Eight neonates with features indistinguishable from AML were studied. Seven patients had Down syndrome and the eighth a normal phenotype. Bone marrow or peripheral blood mononuclear cells (MNC) were cultured in the presence of rhGM-CSF+ rhIL-3 + rhSCF or of rhTPO alone. After incubation, granulocyte-macrophage colony-forming units (CFU-GM)-derived colonies and dusters were scored on an inverted microscope. Colony-forming units-megakaryocyte (CFU-MK)-derived colonies were counted with an in situ CD61 immunostained dish. Liquid suspension cultures of MNC were stimulated by rhGM-CSF and/or rhTPO. Results: CFU-GM-derived colonies and clusters from bone marrow and peripheral blood MNC revealed normal patterns in seven patients. RhTPO-stimulated megakaryocyte colony formation was normal in one patient. Cytospin smears of liquid suspension cultures all showed good myeloid or megakaryocytic maturation consistent with TL rather than AML. One neonate died on the 2nd day of life, but in the seven remaining patients, blasts disappeared from the peripheral blood within 10 months. Among four patients followed longterm, one developed myelodysplastic syndrome at 21 months. This child was given tailored chemotherapy and had a disease-free survival > 20 months. Conclusion: In vitro cell growth stimulated by recombinant human cytokines can help to diagnose TL in neonates.

原文英語
頁(從 - 到)365-371
頁數7
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
106
發行號5
出版狀態已發佈 - 五月 2007
對外發佈Yes

指紋

Thrombopoietin
Granulocyte-Macrophage Progenitor Cells
Newborn Infant
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Acute Myeloid Leukemia
Growth
Stem Cell Factor
Megakaryocytes
Interleukin-3
Blood Cells
Suspensions
Bone Marrow
Myelodysplastic Syndromes
Conditioned Culture Medium
Down Syndrome
Disease-Free Survival
Stem Cells
Cell Culture Techniques
Transient Myeloproliferative Syndrome

ASJC Scopus subject areas

  • Medicine(all)

引用此文

In vitro cell growth stimulated by recombinant human cytokines can help to diagnose transient leukemia in neonates. / Liu, Hsi C.; Chen, Shu Huey; Wang, Lin Y.; Yeh, Ting C.; Chai, I. J.; Liang, Der Cherng.

於: Journal of the Formosan Medical Association = Taiwan yi zhi, 卷 106, 編號 5, 05.2007, p. 365-371.

研究成果: 雜誌貢獻文章

@article{deb5f583760f416a94e5408bb995ab90,
title = "In vitro cell growth stimulated by recombinant human cytokines can help to diagnose transient leukemia in neonates",
abstract = "Background/Purpose: In a previous study, we demonstrated that in vitro cell growth stimulated by human placental conditioned medium distinguished between transient leukemia (TL) and congenital acute myeloid leukemia (AML) in neonates. We then sought to determine whether the application can be expanded if in vitro cell growths are stimulated by recombinant human cytokines including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3), stem cell factor (rhSCF) anal thrombopoietin (rhTPO). Methods: Eight neonates with features indistinguishable from AML were studied. Seven patients had Down syndrome and the eighth a normal phenotype. Bone marrow or peripheral blood mononuclear cells (MNC) were cultured in the presence of rhGM-CSF+ rhIL-3 + rhSCF or of rhTPO alone. After incubation, granulocyte-macrophage colony-forming units (CFU-GM)-derived colonies and dusters were scored on an inverted microscope. Colony-forming units-megakaryocyte (CFU-MK)-derived colonies were counted with an in situ CD61 immunostained dish. Liquid suspension cultures of MNC were stimulated by rhGM-CSF and/or rhTPO. Results: CFU-GM-derived colonies and clusters from bone marrow and peripheral blood MNC revealed normal patterns in seven patients. RhTPO-stimulated megakaryocyte colony formation was normal in one patient. Cytospin smears of liquid suspension cultures all showed good myeloid or megakaryocytic maturation consistent with TL rather than AML. One neonate died on the 2nd day of life, but in the seven remaining patients, blasts disappeared from the peripheral blood within 10 months. Among four patients followed longterm, one developed myelodysplastic syndrome at 21 months. This child was given tailored chemotherapy and had a disease-free survival > 20 months. Conclusion: In vitro cell growth stimulated by recombinant human cytokines can help to diagnose TL in neonates.",
keywords = "Down syndrome, Granulocyte-macrophage colony-stimulating factor, Recombinant human cytokines, Thrombopoietin, Transient leukemia",
author = "Liu, {Hsi C.} and Chen, {Shu Huey} and Wang, {Lin Y.} and Yeh, {Ting C.} and Chai, {I. J.} and Liang, {Der Cherng}",
year = "2007",
month = "5",
language = "English",
volume = "106",
pages = "365--371",
journal = "Journal of the Formosan Medical Association",
issn = "0929-6646",
publisher = "Elsevier Science Publishers B.V.",
number = "5",

}

TY - JOUR

T1 - In vitro cell growth stimulated by recombinant human cytokines can help to diagnose transient leukemia in neonates

AU - Liu, Hsi C.

AU - Chen, Shu Huey

AU - Wang, Lin Y.

AU - Yeh, Ting C.

AU - Chai, I. J.

AU - Liang, Der Cherng

PY - 2007/5

Y1 - 2007/5

N2 - Background/Purpose: In a previous study, we demonstrated that in vitro cell growth stimulated by human placental conditioned medium distinguished between transient leukemia (TL) and congenital acute myeloid leukemia (AML) in neonates. We then sought to determine whether the application can be expanded if in vitro cell growths are stimulated by recombinant human cytokines including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3), stem cell factor (rhSCF) anal thrombopoietin (rhTPO). Methods: Eight neonates with features indistinguishable from AML were studied. Seven patients had Down syndrome and the eighth a normal phenotype. Bone marrow or peripheral blood mononuclear cells (MNC) were cultured in the presence of rhGM-CSF+ rhIL-3 + rhSCF or of rhTPO alone. After incubation, granulocyte-macrophage colony-forming units (CFU-GM)-derived colonies and dusters were scored on an inverted microscope. Colony-forming units-megakaryocyte (CFU-MK)-derived colonies were counted with an in situ CD61 immunostained dish. Liquid suspension cultures of MNC were stimulated by rhGM-CSF and/or rhTPO. Results: CFU-GM-derived colonies and clusters from bone marrow and peripheral blood MNC revealed normal patterns in seven patients. RhTPO-stimulated megakaryocyte colony formation was normal in one patient. Cytospin smears of liquid suspension cultures all showed good myeloid or megakaryocytic maturation consistent with TL rather than AML. One neonate died on the 2nd day of life, but in the seven remaining patients, blasts disappeared from the peripheral blood within 10 months. Among four patients followed longterm, one developed myelodysplastic syndrome at 21 months. This child was given tailored chemotherapy and had a disease-free survival > 20 months. Conclusion: In vitro cell growth stimulated by recombinant human cytokines can help to diagnose TL in neonates.

AB - Background/Purpose: In a previous study, we demonstrated that in vitro cell growth stimulated by human placental conditioned medium distinguished between transient leukemia (TL) and congenital acute myeloid leukemia (AML) in neonates. We then sought to determine whether the application can be expanded if in vitro cell growths are stimulated by recombinant human cytokines including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3), stem cell factor (rhSCF) anal thrombopoietin (rhTPO). Methods: Eight neonates with features indistinguishable from AML were studied. Seven patients had Down syndrome and the eighth a normal phenotype. Bone marrow or peripheral blood mononuclear cells (MNC) were cultured in the presence of rhGM-CSF+ rhIL-3 + rhSCF or of rhTPO alone. After incubation, granulocyte-macrophage colony-forming units (CFU-GM)-derived colonies and dusters were scored on an inverted microscope. Colony-forming units-megakaryocyte (CFU-MK)-derived colonies were counted with an in situ CD61 immunostained dish. Liquid suspension cultures of MNC were stimulated by rhGM-CSF and/or rhTPO. Results: CFU-GM-derived colonies and clusters from bone marrow and peripheral blood MNC revealed normal patterns in seven patients. RhTPO-stimulated megakaryocyte colony formation was normal in one patient. Cytospin smears of liquid suspension cultures all showed good myeloid or megakaryocytic maturation consistent with TL rather than AML. One neonate died on the 2nd day of life, but in the seven remaining patients, blasts disappeared from the peripheral blood within 10 months. Among four patients followed longterm, one developed myelodysplastic syndrome at 21 months. This child was given tailored chemotherapy and had a disease-free survival > 20 months. Conclusion: In vitro cell growth stimulated by recombinant human cytokines can help to diagnose TL in neonates.

KW - Down syndrome

KW - Granulocyte-macrophage colony-stimulating factor

KW - Recombinant human cytokines

KW - Thrombopoietin

KW - Transient leukemia

UR - http://www.scopus.com/inward/record.url?scp=34250688327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250688327&partnerID=8YFLogxK

M3 - Article

C2 - 17561471

AN - SCOPUS:34250688327

VL - 106

SP - 365

EP - 371

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

IS - 5

ER -