In vitro and in vivo anticancer effects of destruxin B on human colorectal cancer

Yi Pang Lee, Che Wei Wang, Wan Chun Liao, Chiung Ru Yang, Chi Tai Yeh, Chung Hung Tsai, Chi Chiang Yang, Yew Min Tzeng

研究成果: 雜誌貢獻文章同行評審

14 引文 斯高帕斯(Scopus)

摘要

Aim: The study of the anticancer effects of destruxin B (DB) is rare and its anticancer mechanism remains unknown. The aim of this study was to test the in vitro and in vivo anticancer effects of DB, on human HT-29 colorectal cancer (CRC). Materials and Methods: DB was isolated and characterized by high pressure liquid chromatography, electrospray ionization mass spectrometry and 1H- nuclear magnetic resonance spectroscopy. (3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used to assess the effects of DB on HT-29 cells in vitro. The anticancer effects of DB were investigated in a murine xenograft model of human colon cancer. Results: A significant inhibition of cell viability was observed with DB treatment in time- and dose-dependent manners. DB administered subcutaneously daily at 0.6-15 mg/kg was proven to be safe and effective in inhibiting the growth of CRC cells. Expression of Bax, cleaved poly (ADP-ribose) polymerase and active caspase-3 were observed with DB treatment and the increase in tumor volumes of treated groups were significantly (p
原文英語
頁(從 - 到)2735-2746
頁數12
期刊Anticancer Research
32
發行號7
出版狀態已發佈 - 七月 2012

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科

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