TY - JOUR
T1 - In vitro activity of colistin sulfate against Enterobacteriaceae producing extended-spectrum β-lactamases
AU - Ku, Yee Huang
AU - Lee, Mei Feng
AU - Chuang, Yin Ching
AU - Chen, Chi Chung
AU - Yu, Wen Liang
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The widespread multidrug-resistant Enterobacteriaceae pose a serious therapeutic challenge. Colistin and tigecycline are potential antimicrobial agents for treating infections caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. We evaluated the in-vitro activity of colistin sulfate against 253 ESBL producers isolated from patients admitted to a medical center in southern Taiwan (Escherichia coli, n = 82; Klebsiella pneumoniae, n = 102; Enterobacter cloacae, n = 34; and Serratia marcescens, n = 35). Colistin showed promising in-vitro activity against E. coli, K. pneumoniae, and E. cloacae, but not S. marcescens. One ESBL-producing K. pneumoniae strain with resistance to carbapenems (ertapenem, imipenem, and meropenem) was selected for time-killing studies. A combination of colistin and tigecycline showed synergism, but there was an inoculum effect. In conclusion, colistin was active against most ESBL-producing Enterobacteriaceae, and a combination of colistin with tigecycline was synergistic against some highly resistant strains, even those with carbapenem resistance.
AB - The widespread multidrug-resistant Enterobacteriaceae pose a serious therapeutic challenge. Colistin and tigecycline are potential antimicrobial agents for treating infections caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. We evaluated the in-vitro activity of colistin sulfate against 253 ESBL producers isolated from patients admitted to a medical center in southern Taiwan (Escherichia coli, n = 82; Klebsiella pneumoniae, n = 102; Enterobacter cloacae, n = 34; and Serratia marcescens, n = 35). Colistin showed promising in-vitro activity against E. coli, K. pneumoniae, and E. cloacae, but not S. marcescens. One ESBL-producing K. pneumoniae strain with resistance to carbapenems (ertapenem, imipenem, and meropenem) was selected for time-killing studies. A combination of colistin and tigecycline showed synergism, but there was an inoculum effect. In conclusion, colistin was active against most ESBL-producing Enterobacteriaceae, and a combination of colistin with tigecycline was synergistic against some highly resistant strains, even those with carbapenem resistance.
KW - Colistin
KW - Enterobacteriaceae
KW - ESBL
KW - Tigecycline
UR - http://www.scopus.com/inward/record.url?scp=84952870818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84952870818&partnerID=8YFLogxK
U2 - 10.1016/j.jmii.2013.11.005
DO - 10.1016/j.jmii.2013.11.005
M3 - Article
C2 - 24388585
AN - SCOPUS:84952870818
VL - 48
SP - 699
EP - 702
JO - Journal of Microbiology, Immunology and Infection
JF - Journal of Microbiology, Immunology and Infection
SN - 0253-2662
IS - 6
ER -