Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence

Chih Yu Wang, Tsuimin Tsai, Chung Ping Chiang, Hsin Ming Chen, Chin Tin Chen

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

We investigate the possibility of using ALA-derived PpIX fluorescence spectroscopy for the detection of epithelial hyperkeratosis (EH) or epithelial dysplasia (ED) lesions in oral submucous fibrosis (OSF) patients that could not be found by autofluorescence spectroscopy. Twenty percent of ALA solution gel was applied onto oral neoplasia and surrounding normal tissue [normal oral mucosa (NOM)] for 90min. Fluorescence emission spectra were measured under 410nm excitation. Generally, the most intense fluorescence emission peaks occurred at 460 and 630nm. The ratios of the area under red peak (630±10nm) to the area under blue peak (460±10nm), denoted as R/B, were calculated. We found that OSF mucosa has the lowest R/B value, followed by NOM, EH on OSF, and ED on OSF. An ANOVA test showed significant differences between OSF, NOM, EH on OSF, and ED on OSF (p0.05). These results indicate that ALA-induced PpIX fluorescence spectroscopy could be used to identify the premalignant lesions on oral fibrotic mucosa, which could not be found by autofluorescence.

原文英語
文章編號044026
期刊Journal of Biomedical Optics
14
發行號4
DOIs
出版狀態已發佈 - 2009

指紋

fibrosis
Aminolevulinic Acid
lesions
Fluorescence
fluorescence
acids
Acids
Fluorescence spectroscopy
Analysis of variance (ANOVA)
spectroscopy
Gels
Mucous Membrane
Spectroscopy
Tissue
emission spectra
gels
excitation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

引用此文

Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence. / Wang, Chih Yu; Tsai, Tsuimin; Chiang, Chung Ping; Chen, Hsin Ming; Chen, Chin Tin.

於: Journal of Biomedical Optics, 卷 14, 編號 4, 044026, 2009.

研究成果: 雜誌貢獻文章

Wang, Chih Yu ; Tsai, Tsuimin ; Chiang, Chung Ping ; Chen, Hsin Ming ; Chen, Chin Tin. / Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence. 於: Journal of Biomedical Optics. 2009 ; 卷 14, 編號 4.
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abstract = "We investigate the possibility of using ALA-derived PpIX fluorescence spectroscopy for the detection of epithelial hyperkeratosis (EH) or epithelial dysplasia (ED) lesions in oral submucous fibrosis (OSF) patients that could not be found by autofluorescence spectroscopy. Twenty percent of ALA solution gel was applied onto oral neoplasia and surrounding normal tissue [normal oral mucosa (NOM)] for 90min. Fluorescence emission spectra were measured under 410nm excitation. Generally, the most intense fluorescence emission peaks occurred at 460 and 630nm. The ratios of the area under red peak (630±10nm) to the area under blue peak (460±10nm), denoted as R/B, were calculated. We found that OSF mucosa has the lowest R/B value, followed by NOM, EH on OSF, and ED on OSF. An ANOVA test showed significant differences between OSF, NOM, EH on OSF, and ED on OSF (p0.05). These results indicate that ALA-induced PpIX fluorescence spectroscopy could be used to identify the premalignant lesions on oral fibrotic mucosa, which could not be found by autofluorescence.",
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