Immunomodulatory effects of hedysarum polybotrys extract in mice macrophages, splenocytes and leucopenia

Guan Cheng Huang, Chia Jung Lee, Kun Teng Wang, Bor Chun Weng, Ting Yi Chien, Sung Hui Tseng, Ching Chiung Wang

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity. Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.
原文英語
頁(從 - 到)14862-14875
頁數14
期刊Molecules
18
發行號12
DOIs
出版狀態已發佈 - 十二月 2013

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

指紋 深入研究「Immunomodulatory effects of hedysarum polybotrys extract in mice macrophages, splenocytes and leucopenia」主題。共同形成了獨特的指紋。

  • 引用此