Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface

Chia Fei Liu, Kai Chun Chang, Ying Sui Sun, Diem Thuy Nguyen, Her Hsiung Huang

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Titanium (Ti) surface, with an essentially bioinert character, is not easy to actively initiate interactions with surrounding biological tissue. In this study, we developed a novel surface modification to enhance the osteogenic response to Ti surface in orthopedic and dental implant applications. The proposed surface modification combined alkaline treatment and type I collagen immobilization through using a natural cross-linker genipin. A variety of experiments were used to characterize the resulting surfaces in terms of morphology, chemistry, roughness, hydrophilicity, and bioactivity. We also assessed the responses of human bone marrow mesenchymal stem cells (hMSCs) to Ti surfaces in terms of adhesion, migration, proliferation, mineralization, and differentiation. Results showed that the modified Ti surfaces revealed a hydrophilic (water and diiodomethane contact angles <22°) nano-/submicron-scaled porous network porosity with surface roughness Ra ~0.1 μm and good bioactivity (Ca/P formation ability). The collagen-immobilized Ti surfaces with good coating adhesion was shown to facilitate the mineralization of the extracellular matrix and the expressions of specific osteogenic markers (osteopontin, bone sialoprotein, and osteocalcin) in hMSCs, thanks to improved cell adhesion and migration. Overall, the proposed Ti implant surface modification proved highly effective in terms of promoting osteogenic responses in vitro.
原文英語
頁(從 - 到)885-900
頁數16
期刊Journal of Materials Research and Technology
15
DOIs
出版狀態已發佈 - 11月 2021

ASJC Scopus subject areas

  • 陶瓷和複合材料
  • 生物材料
  • 表面、塗料和薄膜
  • 金屬和合金

指紋

深入研究「Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface」主題。共同形成了獨特的指紋。

引用此