摘要
We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
原文 | 英語 |
---|---|
頁(從 - 到) | 219-222 |
頁數 | 4 |
期刊 | Nature Methods |
卷 | 4 |
發行號 | 3 |
DOIs | |
出版狀態 | 已發佈 - 三月 2007 |
對外發佈 | Yes |
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Cell Biology