Imaging receptor microdomains on leukocyte subsets in live mice

Elaine Y. Chiang, Andrés Hidalgo, Jungshan Chang, Paul S. Frenette

研究成果: 雜誌貢獻文章同行評審

65 引文 斯高帕斯(Scopus)

摘要

We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

原文英語
頁(從 - 到)219-222
頁數4
期刊Nature Methods
4
發行號3
DOIs
出版狀態已發佈 - 三月 2007
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 分子生物學
  • 細胞生物學

指紋

深入研究「Imaging receptor microdomains on leukocyte subsets in live mice」主題。共同形成了獨特的指紋。

引用此