摘要
We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 219-222 |
| 頁數 | 4 |
| 期刊 | Nature Methods |
| 卷 | 4 |
| 發行號 | 3 |
| DOIs | |
| 出版狀態 | 已發佈 - 三月 2007 |
| 對外發佈 | Yes |
指紋
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Cell Biology
引用此文
Imaging receptor microdomains on leukocyte subsets in live mice. / Chiang, Elaine Y.; Hidalgo, Andrés; Chang, Jungshan; Frenette, Paul S.
於: Nature Methods, 卷 4, 編號 3, 03.2007, p. 219-222.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Imaging receptor microdomains on leukocyte subsets in live mice
AU - Chiang, Elaine Y.
AU - Hidalgo, Andrés
AU - Chang, Jungshan
AU - Frenette, Paul S.
PY - 2007/3
Y1 - 2007/3
N2 - We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
AB - We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
UR - http://www.scopus.com/inward/record.url?scp=33847675756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847675756&partnerID=8YFLogxK
U2 - 10.1038/nmeth1018
DO - 10.1038/nmeth1018
M3 - Article
C2 - 17322889
AN - SCOPUS:33847675756
VL - 4
SP - 219
EP - 222
JO - Nature Methods
JF - Nature Methods
SN - 1548-7091
IS - 3
ER -