Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS

Fu Der Mai, Bo Jung Chen, Li Chen Wu, Feng Yin Li, Wen Kang Chen

研究成果: 雜誌貢獻文章

21 引文 (Scopus)

摘要

Human liver tumor cells intoxicated with five different Cd, Cu, Cr, Hg and Zn metals were analyzed using imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS) to visualize the metal distributions in a single cell basis. A protocol was developed by combining rapid freezing, freeze-fracture and imprinting for transferring the intoxicated cells to a silicon wafer. As shown in the ToF-SIMS images, the cellular morphology was preserved indicating that this protocol can be used to prepare a representative cell for ToF-SIMS imaging analysis. Among the five metal ions investigated in this study, only Cr and Cu ions show preferential diffusion into the cell after simulated intoxication while the signals of the other three ions are either too low to be detected or unable to be distinguished from background intensity.

原文英語
頁(從 - 到)6809-6812
頁數4
期刊Applied Surface Science
252
發行號19
DOIs
出版狀態已發佈 - 七月 30 2006
對外發佈Yes

指紋

heavy metals
Secondary ion mass spectrometry
Heavy Metals
liver
Liver
secondary ion mass spectrometry
Heavy metals
Tumors
tumors
Cells
Imaging techniques
Metals
cells
Ions
intoxication
Silicon wafers
Freezing
Metal ions
metals
freezing

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Condensed Matter Physics

引用此文

Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS. / Mai, Fu Der; Chen, Bo Jung; Wu, Li Chen; Li, Feng Yin; Chen, Wen Kang.

於: Applied Surface Science, 卷 252, 編號 19, 30.07.2006, p. 6809-6812.

研究成果: 雜誌貢獻文章

Mai, Fu Der ; Chen, Bo Jung ; Wu, Li Chen ; Li, Feng Yin ; Chen, Wen Kang. / Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS. 於: Applied Surface Science. 2006 ; 卷 252, 編號 19. 頁 6809-6812.
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title = "Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS",
abstract = "Human liver tumor cells intoxicated with five different Cd, Cu, Cr, Hg and Zn metals were analyzed using imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS) to visualize the metal distributions in a single cell basis. A protocol was developed by combining rapid freezing, freeze-fracture and imprinting for transferring the intoxicated cells to a silicon wafer. As shown in the ToF-SIMS images, the cellular morphology was preserved indicating that this protocol can be used to prepare a representative cell for ToF-SIMS imaging analysis. Among the five metal ions investigated in this study, only Cr and Cu ions show preferential diffusion into the cell after simulated intoxication while the signals of the other three ions are either too low to be detected or unable to be distinguished from background intensity.",
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