Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS

Fu Der Mai, Bo Jung Chen, Li Chen Wu, Feng Yin Li, Wen Kang Chen

研究成果: 雜誌貢獻文章同行評審

26 引文 斯高帕斯(Scopus)


Human liver tumor cells intoxicated with five different Cd, Cu, Cr, Hg and Zn metals were analyzed using imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS) to visualize the metal distributions in a single cell basis. A protocol was developed by combining rapid freezing, freeze-fracture and imprinting for transferring the intoxicated cells to a silicon wafer. As shown in the ToF-SIMS images, the cellular morphology was preserved indicating that this protocol can be used to prepare a representative cell for ToF-SIMS imaging analysis. Among the five metal ions investigated in this study, only Cr and Cu ions show preferential diffusion into the cell after simulated intoxication while the signals of the other three ions are either too low to be detected or unable to be distinguished from background intensity.

頁(從 - 到)6809-6812
期刊Applied Surface Science
出版狀態已發佈 - 七月 30 2006

ASJC Scopus subject areas

  • 化學 (全部)
  • 凝聚態物理學
  • 物理與天文學 (全部)
  • 表面和介面
  • 表面、塗料和薄膜


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