IL-13 expression at the sites of allergen challenge in patients with asthma

S. K. Huang, H. Q. Xiao, J. Kleine-Tebbe, G. Paciotti, D. G. Marsh, L. M. Lichtenstein, M. C. Liu

研究成果: 雜誌貢獻文章

329 引文 (Scopus)

摘要

Atopic asthma is characterized by inflammatory responses of the airway and is associated with up-regulation of Th2 cytokines, notably IL-4 and IL-5. A recently described human cytokine, IL-13, is a potent in vitro modulator of various cell types, including monocytes, B cells, and endothelial cells. Similar to IL-4, it is also involved in the induction of IgE synthesis. However, the in vivo expression and function of IL-13 and its relation to disease remain to be defined. Using a segmental allergen challenge model, we have examined the in vivo expression of IL-13 in the bronchoalveolar lavage (BAL) cells of atopic patients. We found a significant enhancement of both IL-13 transcripts and secreted proteins in the allergen-challenged BAL compared with the saline-challenged control sites of asthmatic and rhinitic patients. In contrast, the expression of IL-13 transcripts was not detected in the BAL of two normal subjects challenged with the same dose of ragweed allergen. The cellular source of IL-13 mRNA was identified in the mononuclear cell fraction of the allergen-challenged BAL. The allergen-induced quantitative differences in the level of transcripts were confirmed by competitive PCR assays. These results suggest that the significant increase in IL-13 in the allergen-challenged BAL is primarily from the mononuclear cells and is involved in the regulation of allergen-induced late phase inflammatory responses.

原文英語
頁(從 - 到)2688-2694
頁數7
期刊Journal of Immunology
155
發行號5
出版狀態已發佈 - 一月 1 1995
對外發佈Yes

指紋

Interleukin-13
Allergens
Asthma
Bronchoalveolar Lavage
Interleukin-4
Cytokines
Ambrosia
Interleukin-5
Immunoglobulin E
Monocytes
B-Lymphocytes
Up-Regulation
Endothelial Cells
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Immunology

引用此文

Huang, S. K., Xiao, H. Q., Kleine-Tebbe, J., Paciotti, G., Marsh, D. G., Lichtenstein, L. M., & Liu, M. C. (1995). IL-13 expression at the sites of allergen challenge in patients with asthma. Journal of Immunology, 155(5), 2688-2694.

IL-13 expression at the sites of allergen challenge in patients with asthma. / Huang, S. K.; Xiao, H. Q.; Kleine-Tebbe, J.; Paciotti, G.; Marsh, D. G.; Lichtenstein, L. M.; Liu, M. C.

於: Journal of Immunology, 卷 155, 編號 5, 01.01.1995, p. 2688-2694.

研究成果: 雜誌貢獻文章

Huang, SK, Xiao, HQ, Kleine-Tebbe, J, Paciotti, G, Marsh, DG, Lichtenstein, LM & Liu, MC 1995, 'IL-13 expression at the sites of allergen challenge in patients with asthma', Journal of Immunology, 卷 155, 編號 5, 頁 2688-2694.
Huang SK, Xiao HQ, Kleine-Tebbe J, Paciotti G, Marsh DG, Lichtenstein LM 等. IL-13 expression at the sites of allergen challenge in patients with asthma. Journal of Immunology. 1995 1月 1;155(5):2688-2694.
Huang, S. K. ; Xiao, H. Q. ; Kleine-Tebbe, J. ; Paciotti, G. ; Marsh, D. G. ; Lichtenstein, L. M. ; Liu, M. C. / IL-13 expression at the sites of allergen challenge in patients with asthma. 於: Journal of Immunology. 1995 ; 卷 155, 編號 5. 頁 2688-2694.
@article{27fa007aaed74251a4f08042ee351ecc,
title = "IL-13 expression at the sites of allergen challenge in patients with asthma",
abstract = "Atopic asthma is characterized by inflammatory responses of the airway and is associated with up-regulation of Th2 cytokines, notably IL-4 and IL-5. A recently described human cytokine, IL-13, is a potent in vitro modulator of various cell types, including monocytes, B cells, and endothelial cells. Similar to IL-4, it is also involved in the induction of IgE synthesis. However, the in vivo expression and function of IL-13 and its relation to disease remain to be defined. Using a segmental allergen challenge model, we have examined the in vivo expression of IL-13 in the bronchoalveolar lavage (BAL) cells of atopic patients. We found a significant enhancement of both IL-13 transcripts and secreted proteins in the allergen-challenged BAL compared with the saline-challenged control sites of asthmatic and rhinitic patients. In contrast, the expression of IL-13 transcripts was not detected in the BAL of two normal subjects challenged with the same dose of ragweed allergen. The cellular source of IL-13 mRNA was identified in the mononuclear cell fraction of the allergen-challenged BAL. The allergen-induced quantitative differences in the level of transcripts were confirmed by competitive PCR assays. These results suggest that the significant increase in IL-13 in the allergen-challenged BAL is primarily from the mononuclear cells and is involved in the regulation of allergen-induced late phase inflammatory responses.",
author = "Huang, {S. K.} and Xiao, {H. Q.} and J. Kleine-Tebbe and G. Paciotti and Marsh, {D. G.} and Lichtenstein, {L. M.} and Liu, {M. C.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "155",
pages = "2688--2694",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - IL-13 expression at the sites of allergen challenge in patients with asthma

AU - Huang, S. K.

AU - Xiao, H. Q.

AU - Kleine-Tebbe, J.

AU - Paciotti, G.

AU - Marsh, D. G.

AU - Lichtenstein, L. M.

AU - Liu, M. C.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Atopic asthma is characterized by inflammatory responses of the airway and is associated with up-regulation of Th2 cytokines, notably IL-4 and IL-5. A recently described human cytokine, IL-13, is a potent in vitro modulator of various cell types, including monocytes, B cells, and endothelial cells. Similar to IL-4, it is also involved in the induction of IgE synthesis. However, the in vivo expression and function of IL-13 and its relation to disease remain to be defined. Using a segmental allergen challenge model, we have examined the in vivo expression of IL-13 in the bronchoalveolar lavage (BAL) cells of atopic patients. We found a significant enhancement of both IL-13 transcripts and secreted proteins in the allergen-challenged BAL compared with the saline-challenged control sites of asthmatic and rhinitic patients. In contrast, the expression of IL-13 transcripts was not detected in the BAL of two normal subjects challenged with the same dose of ragweed allergen. The cellular source of IL-13 mRNA was identified in the mononuclear cell fraction of the allergen-challenged BAL. The allergen-induced quantitative differences in the level of transcripts were confirmed by competitive PCR assays. These results suggest that the significant increase in IL-13 in the allergen-challenged BAL is primarily from the mononuclear cells and is involved in the regulation of allergen-induced late phase inflammatory responses.

AB - Atopic asthma is characterized by inflammatory responses of the airway and is associated with up-regulation of Th2 cytokines, notably IL-4 and IL-5. A recently described human cytokine, IL-13, is a potent in vitro modulator of various cell types, including monocytes, B cells, and endothelial cells. Similar to IL-4, it is also involved in the induction of IgE synthesis. However, the in vivo expression and function of IL-13 and its relation to disease remain to be defined. Using a segmental allergen challenge model, we have examined the in vivo expression of IL-13 in the bronchoalveolar lavage (BAL) cells of atopic patients. We found a significant enhancement of both IL-13 transcripts and secreted proteins in the allergen-challenged BAL compared with the saline-challenged control sites of asthmatic and rhinitic patients. In contrast, the expression of IL-13 transcripts was not detected in the BAL of two normal subjects challenged with the same dose of ragweed allergen. The cellular source of IL-13 mRNA was identified in the mononuclear cell fraction of the allergen-challenged BAL. The allergen-induced quantitative differences in the level of transcripts were confirmed by competitive PCR assays. These results suggest that the significant increase in IL-13 in the allergen-challenged BAL is primarily from the mononuclear cells and is involved in the regulation of allergen-induced late phase inflammatory responses.

UR - http://www.scopus.com/inward/record.url?scp=0029038120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029038120&partnerID=8YFLogxK

M3 - Article

C2 - 7650396

AN - SCOPUS:0029038120

VL - 155

SP - 2688

EP - 2694

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -