IgA immune complex blunts the contraction of cultured mesangial cells through the inhibition of protein kinase C and intracellular calcium

Jong Shiaw Jin, Chen Wen Yao, Shuk Man Ka, Ting Yu Chin, Sheau Heui Chueh, Herng Sheng Lee, Lai Fa Sheu, Yeh Feng Lin, Wei Hwa Lee, Ann Chen

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

The effects of IgA immune complex (IgA-IC) on the contractile function of cultured mesangial cells were measured by the changes in planar surface area in response to treatment with agonists. Incubation of mesangial cells with IgA-IC for 24 hours significantly decreased the contractile responses to angiotensin II (10-6 M) and phorbol 12-myristate 13-acetate (PMA, 10-6 M). Pretreatment of mesangial cells with the protein kinase C (PKC) inhibitor, chelerythrine (10-6 M), eliminated the difference in contractile responses to angiotensin II or PMA between the control and IgA-IC groups indicating IgA-IC may inhibit the activity of PKC. The contractile responses to ionomycin were not significantly different between IgA-IC treated and control mesangial cells, suggesting that the contractile machinery is not impaired by IgA-IC. Intracellular calcium, [Ca2+]i measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells. In contrast, treatment with thapsigargin did not result in significant differences in [Ca2+]i between IgA-IC and control mesangial cells, suggesting that a negligible role of endoplasmic reticulum in the effects of IgA-IC. Using PKC specific antibodies, IgA-IC significantly increased the particulate fraction of PKC-ι of mesangial cells to 141±13% of control, without significantly changing the protein content of PKC-α, -δ and -λ in the cytosolic and particulate fractions. In-summary, IgA-IC inhibits the contractile responses of cultured mesangial cells to agonists by inhibiting the activation of PKC and [Ca2+]i.

原文英語
頁(從 - 到)79-87
頁數9
期刊Chinese Journal of Physiology
47
發行號2
出版狀態已發佈 - 6月 30 2004
對外發佈

ASJC Scopus subject areas

  • 生理學

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