Identifying conserved DR1501-restricted CD4+ T-cell epitopes in avian H5N1 hemagglutinin proteins

Sheng Fan Wang, Ling Yao, Shih Jen Liu, Pele Chong, Wu Tse Liu, Yi Ming Arthur Chen, Jason C. Huang

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Highly pathogenic avian influenza H5N1 viruses are capable of causing poultry epidemics and human mortality. Vaccines that induce protective neutralizing antibodies can prevent outbreaks and decrease the potential for influenza A pandemics. Identifying unique H5N1 virus-specific HLA class II-restricted epitopes is essential for monitoring cellular strain-specific immunity. Our results indicate that 80% of the 30 study participants who were inoculated with an H5N1 vaccine produced neutralizing antibodies. We used intracellular cytokine staining (ICS) to screen and identify six DR1501-restricted H5N1 virus epitopes: H5HA148-162, H5HA 155-169, H5HA253-267, H5HA260-274, H5HA 267-281 and H5HA309-323. Tetramer staining results confirmed that two immunodominant epitopes were DR1501-restricted: H5HA 155-169 and H5HA267-281. Both are located at the HA surface and are highly conserved in currently circulating H5N1 clades. These results suggest that a combination of ICS and tetramer staining can be used as a T-cell epitope-mapping platform, and the identified epitopes may serve as markers for monitoring vaccine efficacy.
原文英語
頁(從 - 到)585-593
頁數9
期刊Viral Immunology
23
發行號6
DOIs
出版狀態已發佈 - 十二月 1 2010
對外發佈Yes

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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