Identification of USF2 as a key regulator of Runx2 expression in mouse pluripotent mesenchymal D1 cells

Chihuei Wang, Grace Lee, Wayne Hsu, Ching Hua Yeh, Mei Ling Ho, Gwo Jaw Wang

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

Runx2 is one of the most important transcription factors directing the osteogenesis of mesenchymal stem cells and osteoblastic functions. It is likely that the factors controlling Runx2 expression would trigger the early steps of osteoblast differentiation. By using a reporter gene assay for 4.5 kb Runx2 promoter, it was found that the first 305 bp of Runx2 promoter are active in D1 cells. Within this region, electromobility shift assays (EMSAs) delineated a 6 bp of CACATG bound specifically by the proteins from D1 cell nuclear extract. Antibody super-shift and DNA-coupling magnetic bead pull-down assay indicated that the protein bound to this sequence is USF2. Site-specific mutagenesis revealed that this sequence contributed to the activity of 305 bp Runx2 promoter. Thus, we suggest that USF2 might be one of the regulators for the expression of the Runx2 gene in D1 cells.

原文英語
頁(從 - 到)79-88
頁數10
期刊Molecular and Cellular Biochemistry
292
發行號1-2
DOIs
出版狀態已發佈 - 十一月 1 2006
對外發佈Yes

指紋

Assays
Site-Directed Mutagenesis
Genes
Cell Extracts
Osteoblasts
Mesenchymal Stromal Cells
Reporter Genes
Osteogenesis
Magnetic couplings
Mutagenesis
Proteins
Transcription Factors
Stem cells
Gene Expression
Antibodies
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

引用此文

Identification of USF2 as a key regulator of Runx2 expression in mouse pluripotent mesenchymal D1 cells. / Wang, Chihuei; Lee, Grace; Hsu, Wayne; Yeh, Ching Hua; Ho, Mei Ling; Wang, Gwo Jaw.

於: Molecular and Cellular Biochemistry, 卷 292, 編號 1-2, 01.11.2006, p. 79-88.

研究成果: 雜誌貢獻文章

Wang, Chihuei ; Lee, Grace ; Hsu, Wayne ; Yeh, Ching Hua ; Ho, Mei Ling ; Wang, Gwo Jaw. / Identification of USF2 as a key regulator of Runx2 expression in mouse pluripotent mesenchymal D1 cells. 於: Molecular and Cellular Biochemistry. 2006 ; 卷 292, 編號 1-2. 頁 79-88.
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AU - Wang, Gwo Jaw

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