Identification of the gene encoding Marek's disease herpesvirus A antigen

R. J. Isfort, H. J. Kung, L. F. Velicer

研究成果: 雜誌貢獻文章

18 引文 (Scopus)

摘要

The gene encoding the glycoprotein Marek's disease herpesvirus A antigen (MDHV-A) precursor polypeptide pr47 was delineated by using Northern blot (RNA blot) analysis and hybrid selection of its mRNA with cloned MDHV DNA, cell-free translation of the mRNA, and immunoprecipitation of the polypeptide. The resulting piece of DNA with strongly positive hybrid selection results was a 2.2-kilobase-pair (kbp) PvuII-EcoRI restriction fragment localized to the center of the 18.3-kbp MDHV BamHI B fragment of the total virus genome. The localization was specific since no other small restriction subfragment of the larger BamHI B fragment was able to hybrid select significant MDHV-A mRNA and the gene mapped only in the BamHI B fragment of the total virus genome. Northern blot analysis confirmed the localization of the MDHV-A gene on the 2.2-kbp fragment and detected its mRNA as a 1.8-kilobase species, a size consistent with encoding a 47-kilodalton polypeptide. This is the first report of an MDHV gene being mapped to the MDHV viral genome. This opens the way for the use of recombinant DNA technology to study the nature of the gene encoding a secreted virus-specific glycoprotein that could possibly be involved in immunoprevention, immunosuppression, or immunoevasion, immune phenomena known or speculated to be involved in this oncogenic herpesvirus system.
原文英語
頁(從 - 到)2614-2620
頁數7
期刊Journal of Virology
61
發行號8
出版狀態已發佈 - 一月 1 1987
對外發佈Yes

指紋

Marek Disease
Marek disease
Herpesviridae
antigens
Antigens
polypeptides
Genes
Viruses
genes
Northern blotting
Northern Blotting
viruses
Messenger RNA
Peptides
genome
glycoproteins
Glycoproteins
Genome
recombinant DNA
Recombinant DNA

ASJC Scopus subject areas

  • Immunology

引用此文

Identification of the gene encoding Marek's disease herpesvirus A antigen. / Isfort, R. J.; Kung, H. J.; Velicer, L. F.

於: Journal of Virology, 卷 61, 編號 8, 01.01.1987, p. 2614-2620.

研究成果: 雜誌貢獻文章

Isfort, RJ, Kung, HJ & Velicer, LF 1987, 'Identification of the gene encoding Marek's disease herpesvirus A antigen', Journal of Virology, 卷 61, 編號 8, 頁 2614-2620.
Isfort, R. J. ; Kung, H. J. ; Velicer, L. F. / Identification of the gene encoding Marek's disease herpesvirus A antigen. 於: Journal of Virology. 1987 ; 卷 61, 編號 8. 頁 2614-2620.
@article{63bba025daed43619d7ac629a06d3bf0,
title = "Identification of the gene encoding Marek's disease herpesvirus A antigen",
abstract = "The gene encoding the glycoprotein Marek's disease herpesvirus A antigen (MDHV-A) precursor polypeptide pr47 was delineated by using Northern blot (RNA blot) analysis and hybrid selection of its mRNA with cloned MDHV DNA, cell-free translation of the mRNA, and immunoprecipitation of the polypeptide. The resulting piece of DNA with strongly positive hybrid selection results was a 2.2-kilobase-pair (kbp) PvuII-EcoRI restriction fragment localized to the center of the 18.3-kbp MDHV BamHI B fragment of the total virus genome. The localization was specific since no other small restriction subfragment of the larger BamHI B fragment was able to hybrid select significant MDHV-A mRNA and the gene mapped only in the BamHI B fragment of the total virus genome. Northern blot analysis confirmed the localization of the MDHV-A gene on the 2.2-kbp fragment and detected its mRNA as a 1.8-kilobase species, a size consistent with encoding a 47-kilodalton polypeptide. This is the first report of an MDHV gene being mapped to the MDHV viral genome. This opens the way for the use of recombinant DNA technology to study the nature of the gene encoding a secreted virus-specific glycoprotein that could possibly be involved in immunoprevention, immunosuppression, or immunoevasion, immune phenomena known or speculated to be involved in this oncogenic herpesvirus system.",
author = "Isfort, {R. J.} and Kung, {H. J.} and Velicer, {L. F.}",
year = "1987",
month = "1",
day = "1",
language = "English",
volume = "61",
pages = "2614--2620",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Identification of the gene encoding Marek's disease herpesvirus A antigen

AU - Isfort, R. J.

AU - Kung, H. J.

AU - Velicer, L. F.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The gene encoding the glycoprotein Marek's disease herpesvirus A antigen (MDHV-A) precursor polypeptide pr47 was delineated by using Northern blot (RNA blot) analysis and hybrid selection of its mRNA with cloned MDHV DNA, cell-free translation of the mRNA, and immunoprecipitation of the polypeptide. The resulting piece of DNA with strongly positive hybrid selection results was a 2.2-kilobase-pair (kbp) PvuII-EcoRI restriction fragment localized to the center of the 18.3-kbp MDHV BamHI B fragment of the total virus genome. The localization was specific since no other small restriction subfragment of the larger BamHI B fragment was able to hybrid select significant MDHV-A mRNA and the gene mapped only in the BamHI B fragment of the total virus genome. Northern blot analysis confirmed the localization of the MDHV-A gene on the 2.2-kbp fragment and detected its mRNA as a 1.8-kilobase species, a size consistent with encoding a 47-kilodalton polypeptide. This is the first report of an MDHV gene being mapped to the MDHV viral genome. This opens the way for the use of recombinant DNA technology to study the nature of the gene encoding a secreted virus-specific glycoprotein that could possibly be involved in immunoprevention, immunosuppression, or immunoevasion, immune phenomena known or speculated to be involved in this oncogenic herpesvirus system.

AB - The gene encoding the glycoprotein Marek's disease herpesvirus A antigen (MDHV-A) precursor polypeptide pr47 was delineated by using Northern blot (RNA blot) analysis and hybrid selection of its mRNA with cloned MDHV DNA, cell-free translation of the mRNA, and immunoprecipitation of the polypeptide. The resulting piece of DNA with strongly positive hybrid selection results was a 2.2-kilobase-pair (kbp) PvuII-EcoRI restriction fragment localized to the center of the 18.3-kbp MDHV BamHI B fragment of the total virus genome. The localization was specific since no other small restriction subfragment of the larger BamHI B fragment was able to hybrid select significant MDHV-A mRNA and the gene mapped only in the BamHI B fragment of the total virus genome. Northern blot analysis confirmed the localization of the MDHV-A gene on the 2.2-kbp fragment and detected its mRNA as a 1.8-kilobase species, a size consistent with encoding a 47-kilodalton polypeptide. This is the first report of an MDHV gene being mapped to the MDHV viral genome. This opens the way for the use of recombinant DNA technology to study the nature of the gene encoding a secreted virus-specific glycoprotein that could possibly be involved in immunoprevention, immunosuppression, or immunoevasion, immune phenomena known or speculated to be involved in this oncogenic herpesvirus system.

UR - http://www.scopus.com/inward/record.url?scp=0023225741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023225741&partnerID=8YFLogxK

M3 - Article

C2 - 3037120

AN - SCOPUS:0023225741

VL - 61

SP - 2614

EP - 2620

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 8

ER -