Identification of the breakage-reunion subunit of T4 DNA topoisomerase

T. C. Rowe, K. M. Tewey, Leroy-Fong Liu

研究成果: 雜誌貢獻文章

67 引文 斯高帕斯(Scopus)

摘要

The antitumor drug 4'-(9-acridinylamino)methanesulfon-m-anisidide which stimulates the cleavable complex formation between mammalian DNA topoisomerase II and DNA also stimulates the cleavable complex formation between bacteriophage T4-induced DNA topoisomerase and DNA. In the presence of 4'-(9-acridinylamino)methanesulfon-m-anisidide, T4 DNA topoisomerase and DNA for a 'cleavable complex' which is characterized by its sensitivity to protein-denaturant treatment. Upon protein-denaturant treatment, the phosphodiester bond of DNA is cleaved, and the gene 52 protein subunit of the topoisomerase becomes covalently linked to the 5'-end of the broken DNA. The covalent protein-DNA linkage has been determined by both paper electrophoresis and thin layer chromatography to be tyrosyl phosphate.

原文英語
頁(從 - 到)9177-9181
頁數5
期刊Journal of Biological Chemistry
259
發行號14
出版狀態已發佈 - 1984
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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