Identification of icp11, the most highly expressed gene of shrimp white spot syndrome virus (WSSV)

Han Ching Wang, Hao Ching Wang, Guang Hsiung Kou, Chu Fang Lo, Wei Pang Huang

研究成果: 雜誌貢獻文章

28 引文 斯高帕斯(Scopus)

摘要

This study investigates white spot syndrome virus (WSSV) gene expression levels in the cells of 2 hosts (Penaeus monodon and Litopenaeus vannamei). Microarray and expressed sequence tag (EST) analysis of the mRNA profiles in WSSV-infected P. monodon cells were used to identify WSSV genes that were very highly expressed. Results showed that the mRNA of the WSSV icp11 gene consistently had the highest copy number of all (3x higher than the major envelope protein, VP28). At the protein level in WSSV-infected L. vannamei, 2-dimensional gel analysis and liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) protein identification also showed that this WSSV non-structural protein has the highest expression levels reported to date. ICP11 is capable of self-multimerization, and it becomes located in both the cytoplasm and nucleus of the host cell. These data suggest that ICP11 plays an important, but presently unknown, role during viral infection, and that expression of the WSSV icp11 gene/WSSV ICP11 protein is potentially a good and diagnostically useful indicator of WSSV infection.
原文英語
頁(從 - 到)179-189
頁數11
期刊Diseases of Aquatic Organisms
74
發行號3
出版狀態已發佈 - 三月 13 2007
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

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