Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo

Chia Che Wu, Erh Hsuan Lin, Yu Ching Lee, Cheng Jeng Tai, Tsu Hsiang Kuo, Hsin Ell Wang, Tsai Yueh Luo, Ying Kai Fu, Haw Jan Chen, Ming Ding Sun, Chih Hsiung Wu, Cheng Wen Wu, Sy Jye Leu, Win Ping Deng

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

A 12-mer amino acid peptide SATTHYRLQAAN, denominated TK4, was isolated from a phage-display library with fibrosarcoma tumor-binding activity. In vivo biodistribution analysis of TK4-displaying phage showed a significant increased phage titer in implanted tumor up to 10-fold in comparison with normal tissues after systemic administration in mouse. Competition assay confirmed that the binding of TK4-phage to tumor cells depends on the TK4 peptide. Intravenous injection of 131I-labeled synthetic TK4 peptide in mice showed a tumor retention of 3.3% and 2.7%ID/g at 1- and 4-hour postinjection, respectively. Tumor-to-muscle ratio was 1.1, 5.7, and 3.2 at 1-, 4-, and 24-hour, respectively, and tumors were imaged on a digital -camera at 4-hour postinjection. The present data suggest that TK4 holds promise as a lead structure for tumor targeting, and it could be further applied in the development of diagnostic or therapeutic agent.

原文英語
文章編號167045
期刊Journal of Biomedicine and Biotechnology
2010
DOIs
出版狀態已發佈 - 2010

指紋

Fibrosarcoma
Tumors
Bacteriophages
Imaging techniques
Peptides
Neoplasms
Digital cameras
Intravenous Injections
Libraries
Muscle
Assays
Display devices
Cells
Tissue
Amino Acids
Muscles

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

引用此文

Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo. / Wu, Chia Che; Lin, Erh Hsuan; Lee, Yu Ching; Tai, Cheng Jeng; Kuo, Tsu Hsiang; Wang, Hsin Ell; Luo, Tsai Yueh; Fu, Ying Kai; Chen, Haw Jan; Sun, Ming Ding; Wu, Chih Hsiung; Wu, Cheng Wen; Leu, Sy Jye; Deng, Win Ping.

於: Journal of Biomedicine and Biotechnology, 卷 2010, 167045, 2010.

研究成果: 雜誌貢獻文章

Wu, Chia Che ; Lin, Erh Hsuan ; Lee, Yu Ching ; Tai, Cheng Jeng ; Kuo, Tsu Hsiang ; Wang, Hsin Ell ; Luo, Tsai Yueh ; Fu, Ying Kai ; Chen, Haw Jan ; Sun, Ming Ding ; Wu, Chih Hsiung ; Wu, Cheng Wen ; Leu, Sy Jye ; Deng, Win Ping. / Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo. 於: Journal of Biomedicine and Biotechnology. 2010 ; 卷 2010.
@article{8344f81449654453b58372d1b5c66a57,
title = "Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo",
abstract = "A 12-mer amino acid peptide SATTHYRLQAAN, denominated TK4, was isolated from a phage-display library with fibrosarcoma tumor-binding activity. In vivo biodistribution analysis of TK4-displaying phage showed a significant increased phage titer in implanted tumor up to 10-fold in comparison with normal tissues after systemic administration in mouse. Competition assay confirmed that the binding of TK4-phage to tumor cells depends on the TK4 peptide. Intravenous injection of 131I-labeled synthetic TK4 peptide in mice showed a tumor retention of 3.3{\%} and 2.7{\%}ID/g at 1- and 4-hour postinjection, respectively. Tumor-to-muscle ratio was 1.1, 5.7, and 3.2 at 1-, 4-, and 24-hour, respectively, and tumors were imaged on a digital -camera at 4-hour postinjection. The present data suggest that TK4 holds promise as a lead structure for tumor targeting, and it could be further applied in the development of diagnostic or therapeutic agent.",
author = "Wu, {Chia Che} and Lin, {Erh Hsuan} and Lee, {Yu Ching} and Tai, {Cheng Jeng} and Kuo, {Tsu Hsiang} and Wang, {Hsin Ell} and Luo, {Tsai Yueh} and Fu, {Ying Kai} and Chen, {Haw Jan} and Sun, {Ming Ding} and Wu, {Chih Hsiung} and Wu, {Cheng Wen} and Leu, {Sy Jye} and Deng, {Win Ping}",
year = "2010",
doi = "10.1155/2010/167045",
language = "English",
volume = "2010",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo

AU - Wu, Chia Che

AU - Lin, Erh Hsuan

AU - Lee, Yu Ching

AU - Tai, Cheng Jeng

AU - Kuo, Tsu Hsiang

AU - Wang, Hsin Ell

AU - Luo, Tsai Yueh

AU - Fu, Ying Kai

AU - Chen, Haw Jan

AU - Sun, Ming Ding

AU - Wu, Chih Hsiung

AU - Wu, Cheng Wen

AU - Leu, Sy Jye

AU - Deng, Win Ping

PY - 2010

Y1 - 2010

N2 - A 12-mer amino acid peptide SATTHYRLQAAN, denominated TK4, was isolated from a phage-display library with fibrosarcoma tumor-binding activity. In vivo biodistribution analysis of TK4-displaying phage showed a significant increased phage titer in implanted tumor up to 10-fold in comparison with normal tissues after systemic administration in mouse. Competition assay confirmed that the binding of TK4-phage to tumor cells depends on the TK4 peptide. Intravenous injection of 131I-labeled synthetic TK4 peptide in mice showed a tumor retention of 3.3% and 2.7%ID/g at 1- and 4-hour postinjection, respectively. Tumor-to-muscle ratio was 1.1, 5.7, and 3.2 at 1-, 4-, and 24-hour, respectively, and tumors were imaged on a digital -camera at 4-hour postinjection. The present data suggest that TK4 holds promise as a lead structure for tumor targeting, and it could be further applied in the development of diagnostic or therapeutic agent.

AB - A 12-mer amino acid peptide SATTHYRLQAAN, denominated TK4, was isolated from a phage-display library with fibrosarcoma tumor-binding activity. In vivo biodistribution analysis of TK4-displaying phage showed a significant increased phage titer in implanted tumor up to 10-fold in comparison with normal tissues after systemic administration in mouse. Competition assay confirmed that the binding of TK4-phage to tumor cells depends on the TK4 peptide. Intravenous injection of 131I-labeled synthetic TK4 peptide in mice showed a tumor retention of 3.3% and 2.7%ID/g at 1- and 4-hour postinjection, respectively. Tumor-to-muscle ratio was 1.1, 5.7, and 3.2 at 1-, 4-, and 24-hour, respectively, and tumors were imaged on a digital -camera at 4-hour postinjection. The present data suggest that TK4 holds promise as a lead structure for tumor targeting, and it could be further applied in the development of diagnostic or therapeutic agent.

UR - http://www.scopus.com/inward/record.url?scp=79251590230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251590230&partnerID=8YFLogxK

U2 - 10.1155/2010/167045

DO - 10.1155/2010/167045

M3 - Article

VL - 2010

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 167045

ER -