Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase

Hei Sheng Huang, Ching Jiunn Chen, Hsieng S. Lu, Wen Chang Chang

研究成果: 雜誌貢獻文章

30 引文 斯高帕斯(Scopus)

摘要

An endogenous lipoxygenase inhibitor, purified from the cytosol of human epidermoid carcinoma A431 cells, was analyzed by N-terminal microsequencing and mass spectrometric analysis. The inhibitor was purified by SDS-PAGE, then subjected to in-gel CNBr cleavage and trypsin digestion. The N-terminal sequence data obtained from a 6-8 kDa band of in-gel CNBr cleavage and the three isolated peptides of in-gel trypsin digestion, and the C-terminal peptide sequence from matrix-assisted laser desorption ionization mass spectrometry matched the sequence of human phospholipid hydroperoxide glutathione peroxidase. The purified inhibitor exhibited peroxidase activity using phosphatidylcholine hydroperoxides as the substrate. We therefore concluded that the lipoxygenase inhibitor present in A431 cells was a phospholipid hydroperoxide glutathione peroxidase.

原文英語
頁(從 - 到)22-26
頁數5
期刊FEBS Letters
424
發行號1-2
DOIs
出版狀態已發佈 - 三月 6 1998
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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