The genomic structure of Tetraodon fluviatilis L29 gene was determined and its promoter activity was analyzed in COS-1 cells and zebrafish embryos. The TfL29 gene comprises four exons and three introns, spanning approximately 1.7kb. The 5′-upstream 2.2-kb of the first exon contains 10 E-boxes and many putative binding motifs for transcription factors GATA-1, AML-1a, c-Myb, Oct-1, CdxA, and NRF-2. Promoter activity assay showed that the distal 2.2-kb fragment not only had high luciferase activity in COS-1 cells, but also strong and ubiquitous GFP expression in a variety of tissues in zebrafish embryos. On the other hand, there are no TATA or CAAT boxes within a 300-bp region upstream from the transcription initiation site. Although this region has high luciferase activity in COS-1 cells, it is not sufficient to drive GFP expression in zebrafish embryos. In this proximal 300-bp region, there are two E-boxes, two CdxA sites, and one NRF-2 site that is immediately downstream of the transcription start site.
|頁（從 - 到）||249-258|
|期刊||Biochemical and Biophysical Research Communications|
|出版狀態||已發佈 - 一月 30 2004|
ASJC Scopus subject areas
- Molecular Biology
Chang, M. H., Chou, C. M., Hsieh, Y. C., Lu, I. C., Devi, M. K. N., Chang, J. P., Kuo, T. F., & Huang, C. J. (2004). Identification of 5′-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish. Biochemical and Biophysical Research Communications, 314(1), 249-258. https://doi.org/10.1016/j.bbrc.2003.12.080