Identification and characterization of dimeric and trimeric circular forms of avian sarcoma virus-specific DNA

Hsing Jien Kung, Peter R. Shank, J. Michael Bishop, Harold E. Varmus

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Covalently closed circular forms of viral DNA are found in the nuclei of cells acutely infected by avian sarcoma virus. In this report we show that dimeric and trimeric forms of closed circular DNA are present, in addition to the previously characterized, predominant monomeric species. The identities of these oligomeric circular molecules were verified by their characteristic behavior during equilibrium centrifugation in cesium chloride-propidium diiodide gradients, rate-zonal centrifugation in sucrose gradients, and electrophoresis in agarose gels, before and after digestion with nucleases. Restriction endonuclease cleavage of isolated dimeric and trimeric molecules indicates that they consist of monomers linked in a "head-to-tail" orientation.
原文英語
頁(從 - 到)425-433
頁數9
期刊Virology
103
發行號2
DOIs
出版狀態已發佈 - 一月 1 1980
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Virology

引用此