Identification and characterization of a hyperglycemic hormone from freshwater giant prawn, Macrobrachium rosenbergii

C. Y. Lin, S. H. Chen, G. H. Kou, C. M. Kuo

研究成果: 雜誌貢獻文章同行評審

17 引文 斯高帕斯(Scopus)

摘要

Crustacean hyperglycemic hormone (CHH), a physiologically important neurohormone stored in the sinus gland of eyestalks, primarily regulates carbohydrate metabolism and also plays significant roles in reproduction, molting and other physiological processes. In the freshwater giant prawn, Macrobrachium rosenbergii, an injection of X-organ sinus gland (XOSG) extract evoked a hyperglycemic response, peaked in 1 h. The hyperglycemic effect of the eyestalk extract was maximal at the dose of 0.5 eyestalk equivalent. CHH fractionated by RP-HPLC, in M. rosenbergii was identified by its hyperglycemic activity and partial amino acid sequence, and the molecular weight of 8534 was determined by matrix-assisted laser desorption ionization mass spectrometry-time of flight analysis (MALDI-TOF). The amino acid sequence of the first 25 residues of CHH showed 72% homology with the first 25 residues of CHH A and CHH B of the American lobster Homarus americanus.

原文英語
頁(從 - 到)315-321
頁數7
期刊Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
121
發行號4
DOIs
出版狀態已發佈 - 十二月 1 1998
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 生理學
  • 分子生物學

指紋

深入研究「Identification and characterization of a hyperglycemic hormone from freshwater giant prawn, Macrobrachium rosenbergii」主題。共同形成了獨特的指紋。

引用此