Hypoxia stimulates the epithelial-to-mesenchymal transition in lung cancer cells through accumulation of nuclear β-catenin

研究成果: 雜誌貢獻文章

摘要

Background/Aim: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. Materials and Methods: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. Results: Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. Conclusion: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process. © 2018 International Institute of Anticancer Research.All right reserved.
原文英語
頁(從 - 到)6299-6308
頁數10
期刊Anticancer Research
38
發行號11
DOIs
出版狀態已發佈 - 十一月 1 2018

指紋

Catenins
Epithelial-Mesenchymal Transition
Lung Neoplasms
Hypoxia-Inducible Factor 1
Snails
Luciferases
Reverse Transcription
Carcinogenesis
Western Blotting
Hypoxia
Polymerase Chain Reaction
Genes

Keywords

  • EMT
  • Epithelial-to-mesenchymal transition
  • Hypoxia
  • Lung cancer
  • β-catenin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此文

@article{760643c250cc41f9ae8829e90cffd3ee,
title = "Hypoxia stimulates the epithelial-to-mesenchymal transition in lung cancer cells through accumulation of nuclear β-catenin",
abstract = "Background/Aim: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. Materials and Methods: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. Results: Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. Conclusion: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process.",
keywords = "EMT, Epithelial-to-mesenchymal transition, Hypoxia, Lung cancer, β-catenin, EMT, Epithelial-to-mesenchymal transition, Hypoxia, Lung cancer, β-catenin",
author = "Liu, {Kao Hui} and Tsai, {Yi Ta} and Chin, {Szu Ying} and Lee, {Woan Ruoh} and Chen, {Yen Chou} and Shen, {Shing Chuan}",
year = "2018",
month = "11",
day = "1",
doi = "10.21873/anticanres.12986",
language = "English",
volume = "38",
pages = "6299--6308",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
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T1 - Hypoxia stimulates the epithelial-to-mesenchymal transition in lung cancer cells through accumulation of nuclear β-catenin

AU - Liu, Kao Hui

AU - Tsai, Yi Ta

AU - Chin, Szu Ying

AU - Lee, Woan Ruoh

AU - Chen, Yen Chou

AU - Shen, Shing Chuan

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Background/Aim: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. Materials and Methods: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. Results: Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. Conclusion: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process.

AB - Background/Aim: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. Materials and Methods: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. Results: Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. Conclusion: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process.

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KW - Lung cancer

KW - β-catenin

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