Hypernegative supercoiling of the DNA template during transcription elongation in vitro

Marc Drolet, Xin Bi, Leroy-Fong Liu

研究成果: 雜誌貢獻文章同行評審

107 引文 斯高帕斯(Scopus)

摘要

Supercoiled plasmid DNAs with negative superhelicity several times higher than normal have been isolated from Escherichia coli topA mutants. The formation of these hypernegatively supercoiled plasmid DNAs is apparently induced by transcription. We show that hypernegatively supercoiled plasmid DNAs isolated from topA mutants contain R-loop(s). To study the mechanism of formation of hypernegatively supercoiled plasmid DNA, we have been able to reproduce hypernegatively supercoiled DNA in vitro using purified DNA polymerase and DNA gyrase. The formation of hypernegatively supercoiled plasmid DNA template in vitro is shown to require transcription elongation and is tightly linked to R-loop formation. We propose that one of the roles of topoisomerase I is to suppress R-loop formation during transcription elongation.

原文英語
頁(從 - 到)2068-2074
頁數7
期刊Journal of Biological Chemistry
269
發行號3
出版狀態已發佈 - 1月 21 1994
對外發佈

ASJC Scopus subject areas

  • 生物化學

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