Hypermodification and immune escape of an internally deleted middle-envelope (M) protein of frequent and predominant hepatitis B virus variants

Pei Ching Tai, Fat Moon Suk, Wolfram H. Gerlich, A. Robert Neurath, Chiaho Shih

研究成果: 雜誌貢獻文章

89 引文 (Scopus)

摘要

Naturally occurring deletions within the human hepatitis B virus (HBV) preS2 region have frequently been identified in patients with hepatocellular carcinoma (HCC), while chronic carriers without cirrhosis and HCC contain no detectable preS2 deletion variants. We have characterized two different preS2 internal deletion variants from two patients. In addition to several weak phenotypes, our study revealed three unexpected strong phenotypes: (1) a paradoxical "hypermodification" phenomenon was observed with significantly increased size heterogeneity and molecular weights of the secreted middle (M) envelope proteins containing a preS2 internal deletion. This phenomenon was observed in transient transfection with a human hepatoma Huh7 cell line as well as in stable transfection with a rodent hepatoma cell line 7777. (2) A significantly increased intracellular accumulation of all three envelope proteins (large, middle, and small) was detected by both Western blot analysis and immunofluorescence microscopy. (3) The middle envelope proteins with a preS2 internal deletion were not recognized in vitro by a putative neutralizing antiserum, suggesting that these variants can evade immune recognition in vivo. To our knowledge, this is the first identification and characterization of the M deletion variant protein in HBV natural infection.

原文英語
頁(從 - 到)44-58
頁數15
期刊Virology
292
發行號1
DOIs
出版狀態已發佈 - 2002

指紋

Hepatitis B virus
Hepatocellular Carcinoma
Transfection
Proteins
Phenotype
Cell Line
Virus Diseases
Fluorescence Microscopy
Immune Sera
Rodentia
Fibrosis
Molecular Weight
Western Blotting

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

引用此文

Hypermodification and immune escape of an internally deleted middle-envelope (M) protein of frequent and predominant hepatitis B virus variants. / Tai, Pei Ching; Suk, Fat Moon; Gerlich, Wolfram H.; Neurath, A. Robert; Shih, Chiaho.

於: Virology, 卷 292, 編號 1, 2002, p. 44-58.

研究成果: 雜誌貢獻文章

Tai, Pei Ching ; Suk, Fat Moon ; Gerlich, Wolfram H. ; Neurath, A. Robert ; Shih, Chiaho. / Hypermodification and immune escape of an internally deleted middle-envelope (M) protein of frequent and predominant hepatitis B virus variants. 於: Virology. 2002 ; 卷 292, 編號 1. 頁 44-58.
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AU - Neurath, A. Robert

AU - Shih, Chiaho

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AB - Naturally occurring deletions within the human hepatitis B virus (HBV) preS2 region have frequently been identified in patients with hepatocellular carcinoma (HCC), while chronic carriers without cirrhosis and HCC contain no detectable preS2 deletion variants. We have characterized two different preS2 internal deletion variants from two patients. In addition to several weak phenotypes, our study revealed three unexpected strong phenotypes: (1) a paradoxical "hypermodification" phenomenon was observed with significantly increased size heterogeneity and molecular weights of the secreted middle (M) envelope proteins containing a preS2 internal deletion. This phenomenon was observed in transient transfection with a human hepatoma Huh7 cell line as well as in stable transfection with a rodent hepatoma cell line 7777. (2) A significantly increased intracellular accumulation of all three envelope proteins (large, middle, and small) was detected by both Western blot analysis and immunofluorescence microscopy. (3) The middle envelope proteins with a preS2 internal deletion were not recognized in vitro by a putative neutralizing antiserum, suggesting that these variants can evade immune recognition in vivo. To our knowledge, this is the first identification and characterization of the M deletion variant protein in HBV natural infection.

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