Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells

I. Tsang Chiang, Wei Ting Chen, Chih Wei Tseng, Yen Chung Chen, Yu Cheng Kuo, Bi Jhih Chen, Mao Chi Weng, Hwai Jeng Lin, Wei Shu Wang

研究成果: 雜誌貢獻文章

22 引文 斯高帕斯(Scopus)

摘要

The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

原文英語
頁(從 - 到)161-167
頁數7
期刊Anticancer Research
37
發行號1
DOIs
出版狀態已發佈 - 一月 1 2017

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

指紋 深入研究「Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells」主題。共同形成了獨特的指紋。

  • 引用此

    Chiang, I. T., Chen, W. T., Tseng, C. W., Chen, Y. C., Kuo, Y. C., Chen, B. J., Weng, M. C., Lin, H. J., & Wang, W. S. (2017). Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells. Anticancer Research, 37(1), 161-167. https://doi.org/10.21873/anticanres.11301