Hypercholesterolemia exacerbates transplant arteriosclerosis via increased neointimal smooth muscle cell accumulation: Studies in apolipoprotein E knockout mice

Chengwei Shi, Wen Sen Lee, Mary E. Russell, Dorothy Zhang, Donald L. Fletcher, John B. Newell, Edgar Haber

研究成果: 雜誌貢獻文章同行評審

30 引文 斯高帕斯(Scopus)

摘要

Background: Hypercholesterolemia is thought to be a significant risk factor for coronary vasculopathy in cardiac transplant recipients. Methods and Results: We examined the development of arteriosclerosis in mouse carotid artery loops allografted from B.10A(2R) (H-2b2) donors to normocholesterolemic C57BL/6J (H-2b) recipients and hypercholesterolemic C57BL/6J recipients in which the apolipoprotein (apo) E gene had been knocked out. Luminal occlusion and cross-sectional neointimal area were greater in arteries allografted into hypercholesterolemic recipients at 15 and 30 days after transplantation. We also measured cellular and extracellular matrix components of the neointima by computerized planimetry of the fractional areas subtended by smooth muscle cells (anti-α-actin stain), collagen (Masson's trichrome), lipid (oil red O), and leukocytes (anti-CD45). The neointimal area stained for smooth muscle cells was significantly greater in hypercholesterolemic recipients than in normocholesterolemic recipients at 15 and 30 days after allografting. Lipid contributed to neointimal area to a lesser degree, and there was no significant increase in the contribution of collagen or leukocytes. Conclusions: Smooth muscle cell accumulation appears to be the principal contributor to the increase in neointimal area observed in arteries allografted into hypercholesterolemic mice.

原文英語
頁(從 - 到)2722-2728
頁數7
期刊Circulation
96
發行號8
出版狀態已發佈 - 10月 21 1997
對外發佈

ASJC Scopus subject areas

  • 生理學
  • 心臟病學與心血管醫學

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