Hydrolysable tannins of tropical almond show antifibrotic effects in TGF-β1-induced hepatic stellate cells

Hsin Ying Chuang, Lean Teik Ng, Liang Tzung Lin, Jung San Chang, Jen Yang Chen, Ta Chen Lin, Chun Ching Lin

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

Background: Persistent activation of hepatic stellate cells (HSC-T6) has been known to cause liver fibrosis. In this study, our objective was to investigate the effects of chebulagic acid and chebulinic acid, two hydrolysable tannins of tropical almond (Terminalia chebula) fruits, on collagen synthesis and signal transduction in transforming growth factor-β1-stimulated HSC-T6 cells. The expression of Smad2, Smad3, Smad4, collagen I(α1)/III, and plasminogen activator inhibitor 1 (PAI-1) mRNAs was determined by reverse-transcription polymerase chain reaction and their protein levels were assessed by western blotting. Results: Results showed that chebulagic acid and chebulinic acid at 20 μmol L -1 exhibited cytotoxic and anti-proliferative effects on HSC-T6 cells. They also significantly decreased the expression of Smd2, Smad3 and Smad4, and the synthesis of collagen, procollagen I (α1) and III, as well as suppressing the activation of PAI-1; these events consequently facilitated the resolution of fibrosis. Conclusion: These results indicate that both chebulagic acid and chebulinic acid possess antifibrotic activity, and their mechanism of action could be through the inhibition of the Smad pathway.

原文英語
頁(從 - 到)2777-2784
頁數8
期刊Journal of the Science of Food and Agriculture
91
發行號15
DOIs
出版狀態已發佈 - 十二月 2011
對外發佈Yes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Nutrition and Dietetics
  • Biotechnology

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