摘要

Background: Brain tissue scarring (gliosis) was believed to be the major cause of epileptic focus after brain injury, and prevention of scarring could reduce the incidence of seizure. We tried the HA coating onto the cortical brain defect of Spraque-Dawley rats to reduce the marginal glial scarring. Methods: A 4 × 2 × 2 mm3 cortical defect was created in the brain of Spraque-Dawley rats. Three percent HA gel was coated onto the lesion for the experimental groups and normal saline solutions for the control groups. The brain was retrieved 4, 8, and 12 weeks after treatment. The brains were then sectioned and processed for H&E and GFAP staining, and the thickness of the scarring and the number of GFAP+ cells were analyzed. Results: The thickness of cutting marginal gliosis was significantly decreased in the HA groups. The 12-week HA group showed the smallest thickness of gliosis, whereas the 12-week control group exhibited the largest thickness of gliosis. The significant difference in the thickness of gliosis was also noted between the HA and the control groups 8 weeks after treatment. The number of GFAP+ cells was also significantly decreased in the HA groups when compared to the respective control group 4, 8, and 12 weeks after the surgery. Conclusion: The results support the hypothesis that HA inhibits glial scarring not only by decreasing the thickness of gliosis but also by reducing the number of the glial cells. Furthermore, our results suggest that HA might be used to reduce glial scar formation in central nervous system surgery, which subsequently prevents the post-operation or posttraumatic seizure incidence.
原文英語
頁(從 - 到)S50-S54
期刊Surgical Neurology
72
發行號SUPPL. 2
DOIs
出版狀態已發佈 - 十二月 2009

指紋

Gliosis
Hyaluronic Acid
Neuroglia
Cicatrix
Brain
Control Groups
Seizures
Cell Count
Incidence
Sodium Chloride
Brain Injuries
Central Nervous System
Gels
Staining and Labeling

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

引用此文

Hyaluronic acid inhibits the glial scar formation after brain damage with tissue loss in rats. / Lin, Chien Min; Lin, Jia Wei; Chen, Yen Chou; Shen, Hsin Hsin; Wei, Li; Yeh, Yi Shian; Chiang, Yung Hsiao; Shih, Raymond; Chiu, Pei Ling; Hung, Kuo Sheng; Yang, Liang Yo; Chiu, Wen Ta.

於: Surgical Neurology, 卷 72, 編號 SUPPL. 2, 12.2009, p. S50-S54.

研究成果: 雜誌貢獻文章

Lin, Chien Min ; Lin, Jia Wei ; Chen, Yen Chou ; Shen, Hsin Hsin ; Wei, Li ; Yeh, Yi Shian ; Chiang, Yung Hsiao ; Shih, Raymond ; Chiu, Pei Ling ; Hung, Kuo Sheng ; Yang, Liang Yo ; Chiu, Wen Ta. / Hyaluronic acid inhibits the glial scar formation after brain damage with tissue loss in rats. 於: Surgical Neurology. 2009 ; 卷 72, 編號 SUPPL. 2. 頁 S50-S54.
@article{51013748e2d741b9828e4f2908d2e35f,
title = "Hyaluronic acid inhibits the glial scar formation after brain damage with tissue loss in rats",
abstract = "Background: Brain tissue scarring (gliosis) was believed to be the major cause of epileptic focus after brain injury, and prevention of scarring could reduce the incidence of seizure. We tried the HA coating onto the cortical brain defect of Spraque-Dawley rats to reduce the marginal glial scarring. Methods: A 4 × 2 × 2 mm3 cortical defect was created in the brain of Spraque-Dawley rats. Three percent HA gel was coated onto the lesion for the experimental groups and normal saline solutions for the control groups. The brain was retrieved 4, 8, and 12 weeks after treatment. The brains were then sectioned and processed for H&E and GFAP staining, and the thickness of the scarring and the number of GFAP+ cells were analyzed. Results: The thickness of cutting marginal gliosis was significantly decreased in the HA groups. The 12-week HA group showed the smallest thickness of gliosis, whereas the 12-week control group exhibited the largest thickness of gliosis. The significant difference in the thickness of gliosis was also noted between the HA and the control groups 8 weeks after treatment. The number of GFAP+ cells was also significantly decreased in the HA groups when compared to the respective control group 4, 8, and 12 weeks after the surgery. Conclusion: The results support the hypothesis that HA inhibits glial scarring not only by decreasing the thickness of gliosis but also by reducing the number of the glial cells. Furthermore, our results suggest that HA might be used to reduce glial scar formation in central nervous system surgery, which subsequently prevents the post-operation or posttraumatic seizure incidence.",
keywords = "Brain injury, Glial scarring, Hyaluronic acid, Posttraumatic epilepsy",
author = "Lin, {Chien Min} and Lin, {Jia Wei} and Chen, {Yen Chou} and Shen, {Hsin Hsin} and Li Wei and Yeh, {Yi Shian} and Chiang, {Yung Hsiao} and Raymond Shih and Chiu, {Pei Ling} and Hung, {Kuo Sheng} and Yang, {Liang Yo} and Chiu, {Wen Ta}",
year = "2009",
month = "12",
doi = "10.1016/j.wneu.2009.09.004",
language = "English",
volume = "72",
pages = "S50--S54",
journal = "World Neurosurgery",
issn = "1878-8750",
publisher = "Elsevier Inc.",
number = "SUPPL. 2",

}

TY - JOUR

T1 - Hyaluronic acid inhibits the glial scar formation after brain damage with tissue loss in rats

AU - Lin, Chien Min

AU - Lin, Jia Wei

AU - Chen, Yen Chou

AU - Shen, Hsin Hsin

AU - Wei, Li

AU - Yeh, Yi Shian

AU - Chiang, Yung Hsiao

AU - Shih, Raymond

AU - Chiu, Pei Ling

AU - Hung, Kuo Sheng

AU - Yang, Liang Yo

AU - Chiu, Wen Ta

PY - 2009/12

Y1 - 2009/12

N2 - Background: Brain tissue scarring (gliosis) was believed to be the major cause of epileptic focus after brain injury, and prevention of scarring could reduce the incidence of seizure. We tried the HA coating onto the cortical brain defect of Spraque-Dawley rats to reduce the marginal glial scarring. Methods: A 4 × 2 × 2 mm3 cortical defect was created in the brain of Spraque-Dawley rats. Three percent HA gel was coated onto the lesion for the experimental groups and normal saline solutions for the control groups. The brain was retrieved 4, 8, and 12 weeks after treatment. The brains were then sectioned and processed for H&E and GFAP staining, and the thickness of the scarring and the number of GFAP+ cells were analyzed. Results: The thickness of cutting marginal gliosis was significantly decreased in the HA groups. The 12-week HA group showed the smallest thickness of gliosis, whereas the 12-week control group exhibited the largest thickness of gliosis. The significant difference in the thickness of gliosis was also noted between the HA and the control groups 8 weeks after treatment. The number of GFAP+ cells was also significantly decreased in the HA groups when compared to the respective control group 4, 8, and 12 weeks after the surgery. Conclusion: The results support the hypothesis that HA inhibits glial scarring not only by decreasing the thickness of gliosis but also by reducing the number of the glial cells. Furthermore, our results suggest that HA might be used to reduce glial scar formation in central nervous system surgery, which subsequently prevents the post-operation or posttraumatic seizure incidence.

AB - Background: Brain tissue scarring (gliosis) was believed to be the major cause of epileptic focus after brain injury, and prevention of scarring could reduce the incidence of seizure. We tried the HA coating onto the cortical brain defect of Spraque-Dawley rats to reduce the marginal glial scarring. Methods: A 4 × 2 × 2 mm3 cortical defect was created in the brain of Spraque-Dawley rats. Three percent HA gel was coated onto the lesion for the experimental groups and normal saline solutions for the control groups. The brain was retrieved 4, 8, and 12 weeks after treatment. The brains were then sectioned and processed for H&E and GFAP staining, and the thickness of the scarring and the number of GFAP+ cells were analyzed. Results: The thickness of cutting marginal gliosis was significantly decreased in the HA groups. The 12-week HA group showed the smallest thickness of gliosis, whereas the 12-week control group exhibited the largest thickness of gliosis. The significant difference in the thickness of gliosis was also noted between the HA and the control groups 8 weeks after treatment. The number of GFAP+ cells was also significantly decreased in the HA groups when compared to the respective control group 4, 8, and 12 weeks after the surgery. Conclusion: The results support the hypothesis that HA inhibits glial scarring not only by decreasing the thickness of gliosis but also by reducing the number of the glial cells. Furthermore, our results suggest that HA might be used to reduce glial scar formation in central nervous system surgery, which subsequently prevents the post-operation or posttraumatic seizure incidence.

KW - Brain injury

KW - Glial scarring

KW - Hyaluronic acid

KW - Posttraumatic epilepsy

UR - http://www.scopus.com/inward/record.url?scp=71549139718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549139718&partnerID=8YFLogxK

U2 - 10.1016/j.wneu.2009.09.004

DO - 10.1016/j.wneu.2009.09.004

M3 - Article

VL - 72

SP - S50-S54

JO - World Neurosurgery

JF - World Neurosurgery

SN - 1878-8750

IS - SUPPL. 2

ER -