Human T-cell responses to ragweek allergens: Amb V homologues

S. K. Huang, D. G. Marsh

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

Specific IgE and IgG responses to highly purified Ambrosia (ragweed) allergens, Amb a V, Amb t V and Amb p V from the artemisiifolia (short), trifida (giant) and psilostachya (western) species are strongly associated with HLA-DR2 and Dw2 (DR2.2) in allergic Caucasoid individuals. To investigate the molecular basis of these HLA associations, we examined the human T-cell responses to these Amb V homologues using three Amb a V-specific, DRαβ(I)2.2-restricted T-cell clones from an atopic patient. We first examined the cross-reactivity of Amb a V-specific T-cell clones upon challenge with the Amb a V homologues, Amb t V and Amb p V, in the presence of autologous antigen-presenting cells (APC). Neither Amb t V nor Amb p V was able to stimulate the T-cell clones directly. However, both Abm t V and Amb p V specifically blocked, in a dose-dependent fashion, the ability of APC to present Amb a V to all thre T-cell clones. Taken together, these results suggest that Amb t V and Amb p V possess distinct T-cell epitopes, but that all Amb V homologues share similar or identical regions (agretopes) interacting with the DR2αβ(I)2.2 (DRαβ(I)1501) heterodimer. The agretope was potentially localized to a 14-residue C-terminal Amb a V peptide (with Ala-Cys substitutions), which waws able to block presentation of native Amb a V by the APC to the T-cell clones.

原文英語
頁(從 - 到)363-365
頁數3
期刊Immunology
73
發行號3
出版狀態已發佈 - 一月 1 1991
對外發佈Yes

指紋

Allergens
T-Lymphocytes
Clone Cells
Antigen-Presenting Cells
Ambrosia
HLA-DR2 Antigen
T-Lymphocyte Epitopes
Autoantigens
Immunoglobulin E
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology

引用此文

Human T-cell responses to ragweek allergens : Amb V homologues. / Huang, S. K.; Marsh, D. G.

於: Immunology, 卷 73, 編號 3, 01.01.1991, p. 363-365.

研究成果: 雜誌貢獻文章

Huang, SK & Marsh, DG 1991, 'Human T-cell responses to ragweek allergens: Amb V homologues', Immunology, 卷 73, 編號 3, 頁 363-365.
Huang, S. K. ; Marsh, D. G. / Human T-cell responses to ragweek allergens : Amb V homologues. 於: Immunology. 1991 ; 卷 73, 編號 3. 頁 363-365.
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