Human platelet lysate

Replacing fetal bovine serum as a gold standard for human cell propagation?

Thierry Burnouf, Dirk Strunk, Mickey B C Koh, Katharina Schallmoser

研究成果: 雜誌貢獻回顧型文獻

120 引文 (Scopus)

摘要

The essential physiological role of platelets in wound healing and tissue repair builds the rationale for the use of human platelet derivatives in regenerative medicine. Abundant growth factors and cytokines stored in platelet granules can be naturally released by thrombin activation and clotting or artificially by freeze/thaw-mediated platelet lysis, sonication or chemical treatment. Human platelet lysate prepared by the various release strategies has been established as a suitable alternative to fetal bovine serum as culture medium supplement, enabling efficient propagation of human cells under animal serum-free conditions for a multiplicity of applications in advanced somatic cell therapy and tissue engineering. The rapidly increasing number of studies using platelet derived products for inducing human cell proliferation and differentiation has also uncovered a considerable variability of human platelet lysate preparations which limits comparability of results. The main variations discussed herein encompass aspects of donor selection, preparation of the starting material, the possibility for pooling in plasma or additive solution, the implementation of pathogen inactivation and consideration of ABO blood groups, all of which can influence applicability. This review outlines the current knowledge about human platelet lysate as a powerful additive for human cell propagation and highlights its role as a prevailing supplement for human cell culture capable to replace animal serum in a growing spectrum of applications.
原文英語
頁(從 - 到)371-387
頁數17
期刊Biomaterials
76
DOIs
出版狀態已發佈 - 2016

指紋

Platelets
Blood Platelets
Cells
Serum
Cell culture
Animals
Cell Engineering
Donor Selection
Sonication
Regenerative Medicine
Cell proliferation
Pathogens
Tissue Engineering
Blood Group Antigens
Cell- and Tissue-Based Therapy
Tissue engineering
Thrombin
Wound Healing
Culture Media
Cell Differentiation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

引用此文

Human platelet lysate : Replacing fetal bovine serum as a gold standard for human cell propagation? / Burnouf, Thierry; Strunk, Dirk; Koh, Mickey B C; Schallmoser, Katharina.

於: Biomaterials, 卷 76, 2016, p. 371-387.

研究成果: 雜誌貢獻回顧型文獻

Burnouf, Thierry ; Strunk, Dirk ; Koh, Mickey B C ; Schallmoser, Katharina. / Human platelet lysate : Replacing fetal bovine serum as a gold standard for human cell propagation?. 於: Biomaterials. 2016 ; 卷 76. 頁 371-387.
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