Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR

Ching Yu Lin, Angel Chao, Yuh Cheng Yang, Hung Hsueh Chou, Chih Ming Ho, Ruey Wen Lin, Ting Chang Chang, Jia Yia Chiou, Fang Yu Chao, Kung Liahng Wang, Tsai Yen Chien, Swei Hsueh, Chu Chun Huang, Chien Jen Chen, Chyong Huey Lai

研究成果: 雜誌貢獻文章

31 引文 斯高帕斯(Scopus)

摘要

Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip® HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen's κ = 0.93 (95% CI: 0.90-0.97) and McNemar's test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0% (394/433). Sensitivity (90-100%), specificity (99.2-100%), and accuracy (98.6-100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.
原文英語
頁(從 - 到)361-367
頁數7
期刊Journal of Clinical Virology
42
發行號4
DOIs
出版狀態已發佈 - 八月 2008

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

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    Lin, C. Y., Chao, A., Yang, Y. C., Chou, H. H., Ho, C. M., Lin, R. W., Chang, T. C., Chiou, J. Y., Chao, F. Y., Wang, K. L., Chien, T. Y., Hsueh, S., Huang, C. C., Chen, C. J., & Lai, C. H. (2008). Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR. Journal of Clinical Virology, 42(4), 361-367. https://doi.org/10.1016/j.jcv.2008.03.018