Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation

Tzu Yang Weng, Hsuan Franziska Wu, Chung Yen Li, Yu Hsuan Hung, Yu Wei Chang, Yi Ling Chen, Hui Ping Hsu, Yu Hung Chen, Chih Yang Wang, Jang Yang Chang, Ming Derg Lai

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Homoharringtonine (HHT), an inhibitor of protein synthesis, has been used to treat leukemia. Its therapeutic effects on non-small cell lung adenocarcinoma carrying KRAS mutation and their immune system are less understood. The present study examined the therapeutic efficacy and the immune effects of HHT in two murine lung tumor models, xenograft and transgenic, carrying the Kras mutation G12D and G12C respectively. HHT exhibited efficient anticancer activity, significantly suppressing lung tumor growth in vitro and in vivo. The levels of 22 cytokines and chemokines in splenocytes of tumor-bearing mice were examined. Interleukin-12 expression was lower in splenocytes of HHT-treated mice when compared to the controls as demonstrated by a cytokine array and an enzyme-linked immunosorbent assay. The expression levels of CD80, CD86, and CD69 in B220+ B cells from splenocytes of HHT-treated mice were higher than that of control mice in two mouse tumor models. Furthermore, antitumor effect of HHT was attenuated with depletion of B cells. Increased numbers of CD80+ and CD86+ B cells were observed in the mice treated with narciclasine, another translation inhibitor. In conclusion, HHT changed the features of immune cells, and exhibited efficient anti-tumor activity against lung tumor carrying mutant Kras expression.
原文英語
文章編號8216
期刊Scientific Reports
8
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2018
對外發佈Yes

ASJC Scopus subject areas

  • General

指紋 深入研究「Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation」主題。共同形成了獨特的指紋。

  • 引用此