Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal

Yu Cheng Hsiao, Yu Chien Sung, Mon Juan Lee, Wei Lee

研究成果: 雜誌貢獻文章同行評審

33 引文 斯高帕斯(Scopus)

摘要

Liquid crystal (LC)-based biosensors employ highly sensitive interfaces between the alignment layers and LCs to detect biomolecules and their interactions. Present techniques based on optical texture observation of the homeotropic-to-planar response of nematic LCs are limited by their quantitative reproducibility of results, indicating that both the accuracy and reliability of LC-based detection require further improvements. Here we show that cholesteric LC (CLC) can be used as a novel sensing element in the design of an alternative LC-based biosensing device. The chirality of the vertically anchored (VA) CLC was exploited in the detection of bovine serum albumin (BSA), a protein standard commonly used in protein quantitation. The color appearance and the corresponding transmission spectrum of the cholesteric phase changed with the concentration of BSA, by which a detection limit of 1 fg/ml was observed. The optical response of the VA CLC interface offers a simple and inexpensive platform for highly sensitive and naked-eye color-indicating detection of biomolecules, and, thus, may facilitate the development of point-of-care devices for the detection of disease-related biomarkers.
原文英語
文章編號250493
頁(從 - 到)5033-5038
頁數6
期刊Biomedical Optics Express
6
發行號12
DOIs
出版狀態已發佈 - 十一月 23 2015
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 原子與分子物理與光學

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