Highly efficient fluorescent QDs sensor for specific detection of protein through double recognition of hybrid aptamer-molecular imprinted polymers

Jiean Tan, Manli Guo, Lei Tan, Yuanyuan Geng, Shuyi Huang, Youwen Tang, Chaochin Su, Chun Che Lin, Yong Liang

研究成果: 雜誌貢獻文章同行評審

33 引文 斯高帕斯(Scopus)

摘要

This work demonstrates a strategy of double recognition hybrid aptamer–molecular imprinted polymer/quantum dots (apt–MIP/QDs) fluorescent sensor for specific recognition of target protein. We selected vinyl modified Mn–doped ZnS QDs as signal source and support. Instead of relying on single source of weak interaction between protein and small molecule monomer, thiol terminated aptamers were used as specific functional monomers to obtain apt–MIP on the surface of QDs. The double recognition was based on aptamer and imprinted cavity to overcome the challenges of protein imprinting. The synergistic effect of the aptamer and MAA contributed for highly specific recognition ability of the apt–MIP/QDs sensor toward Cyt C. The imprinting factor and the selectivity factor values of the hybrid apt–MIP/QDs sensor were 3.17 and 8.33, respectively. This composite sensor showed an excellent linear correlation for Cyt C in the concentration range of 0.20–2.00 μM. The detection limit for Cyt C was 0.054 μM A sensitive, rapid and convenient recognition system for Cyt C in biological samples was developed. The recoveries were between 94.2% and 102.5% for urine and serum samples.
原文英語
頁(從 - 到)627-635
頁數9
期刊Sensors and Actuators, B: Chemical
274
DOIs
出版狀態已發佈 - 十一月 20 2018
對外發佈

ASJC Scopus subject areas

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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