High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens

Yu Jui Fan, Yu Chun Kung, Yi Chien Wu, Kuo Wei Huang, Ting Hsiang Wu, Yue Chen, Horn Jiunn Sheen, Pei Yu Chiou

研究成果: 書貢獻/報告類型會議貢獻

2 引文 (Scopus)

摘要

We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS structures with vertical vias connecting fluid in different layers. An array of high N.A. microball lens is also embedded in the structure to achieve parallel and high sensitivity fluorescence detection across these 32 channels.
原文英語
主出版物標題Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
發行者Chemical and Biological Microsystems Society
頁面1909-1911
頁數3
ISBN(列印)9780979806452
出版狀態已發佈 - 2012
對外發佈Yes
事件16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, 日本
持續時間: 十月 28 2012十一月 1 2012

會議

會議16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
國家日本
城市Okinawa
期間10/28/1211/1/12

指紋

Microfluidics
Fluorescence
Throughput
Microfabrication
Lithography
Lenses
Multilayers
Fluids

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

引用此文

Fan, Y. J., Kung, Y. C., Wu, Y. C., Huang, K. W., Wu, T. H., Chen, Y., ... Chiou, P. Y. (2012). High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (頁 1909-1911). Chemical and Biological Microsystems Society.

High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. / Fan, Yu Jui; Kung, Yu Chun; Wu, Yi Chien; Huang, Kuo Wei; Wu, Ting Hsiang; Chen, Yue; Sheen, Horn Jiunn; Chiou, Pei Yu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1909-1911.

研究成果: 書貢獻/報告類型會議貢獻

Fan, YJ, Kung, YC, Wu, YC, Huang, KW, Wu, TH, Chen, Y, Sheen, HJ & Chiou, PY 2012, High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 頁 1909-1911, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 日本, 10/28/12.
Fan YJ, Kung YC, Wu YC, Huang KW, Wu TH, Chen Y 等. High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1909-1911
Fan, Yu Jui ; Kung, Yu Chun ; Wu, Yi Chien ; Huang, Kuo Wei ; Wu, Ting Hsiang ; Chen, Yue ; Sheen, Horn Jiunn ; Chiou, Pei Yu. / High throughput fluorescence based flow cytometer using 3D microfluidics for parallel sheath flow focusing and embeded high N.A. Microlens. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. 頁 1909-1911
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abstract = "We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS structures with vertical vias connecting fluid in different layers. An array of high N.A. microball lens is also embedded in the structure to achieve parallel and high sensitivity fluorescence detection across these 32 channels.",
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AU - Fan, Yu Jui

AU - Kung, Yu Chun

AU - Wu, Yi Chien

AU - Huang, Kuo Wei

AU - Wu, Ting Hsiang

AU - Chen, Yue

AU - Sheen, Horn Jiunn

AU - Chiou, Pei Yu

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AB - We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS structures with vertical vias connecting fluid in different layers. An array of high N.A. microball lens is also embedded in the structure to achieve parallel and high sensitivity fluorescence detection across these 32 channels.

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KW - Flow cytometry

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