High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Jin Ching Lee, Tzong Yuan Wu, Chien Fu Huang, Feng Mine Yang, Shin Ru Shih, John T.A. Hsu

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.
原文英語
頁(從 - 到)656-662
頁數7
期刊Biotechnology and Bioengineering
90
發行號5
DOIs
出版狀態已發佈 - 六月 5 2005
對外發佈Yes

指紋

Genetic Enhancement
Encephalomyocarditis virus
Recombinant Fusion Proteins
Protein Engineering
Genetic engineering
Genetic Engineering
CHO Cells
Enterovirus
COS Cells
Protein Biosynthesis
Cricetulus
Tumor Cell Line
RNA
Ribosomes
Viruses
Cricetinae
Transfection
Hepatocellular Carcinoma
Animals
Enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

引用此文

High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site. / Lee, Jin Ching; Wu, Tzong Yuan; Huang, Chien Fu; Yang, Feng Mine; Shih, Shin Ru; Hsu, John T.A.

於: Biotechnology and Bioengineering, 卷 90, 編號 5, 05.06.2005, p. 656-662.

研究成果: 雜誌貢獻文章

Lee, Jin Ching ; Wu, Tzong Yuan ; Huang, Chien Fu ; Yang, Feng Mine ; Shih, Shin Ru ; Hsu, John T.A. / High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site. 於: Biotechnology and Bioengineering. 2005 ; 卷 90, 編號 5. 頁 656-662.
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AU - Hsu, John T.A.

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